Antibody array used for the analysis of the three-dimensional structure of protein therapeutics and its production
AssigneeArray Bridge Inc.
InventorXing Wang
Life of this patent
Estimating the term…
Abstract
This disclosure provides an antibody array for the analysis of the three-dimensional structure of a protein. It includes the development and production of the antibody array and methods of using the array to analyze the three-dimensional structure of a protein as well as to compare the three-dimensional structure of two proteins, for example, a therapeutic protein and a biosimilar, to determine if the two proteins are similar.
Claims17
A method of determining the conformational or 3-dimensional structure of a protein comprising:
- (a) contacting said protein with an array of antibodies that specifically bind to epitopes that cover the surface of said protein;
- and
- (b) measuring the quantity of complex formed between each antibody specifically bound to said protein;
- wherein the quantity of complex formed between each of the antibodies of the array and the protein is used to determine the conformational or 3-dimensional structure of the protein.
A method of comparing the conformational or 3-dimensional structure of a first protein and a second protein comprising:
- contacting each of said first and second proteins with an array of antibodies that specifically bind to epitopes that cover the surface of said first and second proteins, respectively;
- measuring the quantity of complex formed between each antibody specifically bound to said first and second proteins;
- and comparing the quantity of complex formed between each antibody specifically bound to said first and second proteins, wherein the conformational or 3-dimensional structures of said first protein and said second protein are similar if the quantity of complex formed between each antibody specifically bound to said first protein is similar to the quantity of complex formed between each antibody specifically bound to said second protein.
Dependencies are inferred from the claim text.