Method for producing polyphenol compounds
AssigneesUniversite Pierre Et Marie Curie (Paris 6), Centre National de la Recherche Scientifique (CNRS)
InventorsLudovic Delage, Laurence Meslet-Cladiere, Philippe Potin, Sophie Goulitquer
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Abstract
The present invention relates to a method for producing polyphenol compounds, i.e. phloroglucinol or one of its derivatives, with a polyketide synthase of type III (PKSIII) from a brown marine alga. The invention also relates to recombinant nucleic acids coding for a polyketide synthase of type III (PKSIII) from the brown alga Ectocarpus siliculosus (E. siliculosus), to recombinant vectors comprising these nucleic acids, as well as to host cells comprising these vectors. Finally, the invention relates to a method for preparing of various compounds by means of polyphenol compounds produced according to the aforementioned method.
Claims3
A method for producing at least one polyphenol compound, comprising:
- contacting a polyketide synthase of type III (PKSIII) from brown algae of the Ectocarpus genus with at least one carbonaceous substrate chosen from malonyl-CoA, acetyl-CoA, hexanoyl-CoA, decanoyl-CoA, lauroyl-CoA, and/or palmitoyl-CoA, under conditions allowing production of at least one polyphenol compound, wherein said polyphenol compound is phloroglucinol or an acyl-phloroglucinol, wherein said PKSIII comprises:
- the amino acid sequence of SEQ ID NO:
- 1;
- an amino acid sequence having at least 93% identity with the amino acid sequence of SEQ ID NO:
- 1;
- and
- a tag sequence coding for a repetition of histidines before or after the coding sequence of said PKSIII.
Dependencies are inferred from the claim text.