Microorganisms for biosynthesis of limonene on gaseous substrates
AssigneeKiverdi, Inc.
InventorsChrister Jansson, Cody A. Marcus Carr, John S. Reed
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Abstract
Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.
Claims26
A composition comprising a non-naturally occurring knallgas microorganism that is capable of growing on a gaseous substrate as a carbon and energy source, and a culture medium in contact with a gaseous substrate that comprises:
- an inorganic carbon source comprising CO2 and/or CO;
- an inorganic gaseous electron donor comprising H2 and/or CO;
- and an inorganic gaseous electron acceptor comprising O2, wherein the gaseous substrate comprises at least 2% O2,
- wherein said microorganism is a Cupriavidus sp. or Ralstonia sp. or Hydrogenobacter sp. that comprises at least one exogenous nucleic acid encoding an efflux pump and/or comprises the ability to overexpress a native efflux pump, and
- wherein the gaseous substrate is utilized by said microorganism for production of a terpene in the culture medium using a combination of the electron donor and the electron acceptor as an energy source.
Dependencies are inferred from the claim text.