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US 10,179,898 B2Granted
Bioreactor for the cultivation of mammalian cells
AssigneeLonza Biologics PLC
InventorMohsan Khan
Life of this patent
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Abstract
The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
Claims17
A scalable bioreactor for the cultivation of mammalian cells, the bioreactor comprising:
- a biocompatible tank or vessel having a volume of between 500 liters and 20,000 liters and at least one top impeller and at least one bottom impeller,
- wherein the at least one top impeller has a power number (Np) between 0.1 and 0.9,
- wherein the at least one top impeller has a flow number (Ng) between 0.4 and 0.9,
- wherein the at least one bottom impeller has a power number (Np) between 0.5 and 0.9,
- wherein an impeller spacing (Ds) between the at least one top impeller and the at least one bottom impeller is between 1.229×the diameter of the bottom impeller (Dbottom) and 2×Dbottom,
- wherein the height of a liquid above the at least one top impeller (Do) is between 0.3×the diameter of the at least one top impeller (Dtop) and 2.5×Dtop, and
- wherein a bottom clearance (Dc) between the tank bottom and the center-line of the bottom impeller is at least 0.35×Dbottom.
4 more elements
Dependencies are inferred from the claim text.