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US 10,179,822 B2Granted
Humanized monoclonal antibodies and methods of use
AssigneeDana-Farber Cancer Institute, Inc.
InventorsWayne A. Marasco, Jianhua Sui, Yuval Avnir
Life of this patent
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Abstract
The present invention comprises a humanized monoclonal antibody that binds to the human immunoglobulin heavy chain variable region germline gene VH1-69. This antibody is derived from Mab G6 and recognizes the same epitope. Moreover, the antibody is used in combination with vaccines to augment an immune response to the antigen.
Claims24
An isolated humanized monoclonal antibody, wherein said antibody comprises at least one of:
- a. a heavy chain variable region encoded by the nucleic acid sequence comprising:
- (SEQ ID NO:
- 1)
- CAGGTCCAGCTCGTCCAGTCCGGCGCTGAAGTGGTGAAACCCGGGGCATC
- CGTCAAAGTCTCTTGTAAGGCTAGTGGCTACACCTTCACATCCTACTGGA
- TGCATTGGGTGAAACAGGCACCTGGCCAGGGACTCGAATGGATCGGAGCC
- GTGTCTCCTGGAAATTCCGACACCTCCTACAACGAAAAATTCAAGGGCAA
- GGCAACCCTCACTGTGGATACTAGTGCTTCTACCGCCTACATGGAACTCT
- CATCTCTCCGCTCTGAGGACACTGCCGTCTACTACTGTACTCGGTCACGA
- TACGGGAACAACGCTCTCGATTACTGGGGACAGGGCACACTGGTCACTGT
- CTCT;
- b. a heavy chain variable region comprising the amino acid sequence:
- (SEQ ID NO:
- 2)
- QVQLVQSGAEVVKPGASVKVSCKASGYTFTSYWMHWVKQAPGQGLEWIGA
- VSPGNSDTSYNEKFKGKATLTVDTSASTAYMELSSLRSEDTAVYYCTRSR
- YGNNALDYWGQGTLVTVS;
- c. a light chain variable region encoded by the nucleic acid sequence comprising:
- (SEQ ID NO:
- 3)
- GATATTCAGCTCACACAGAGCCCATCTTCTCTGTCTGCTTCTGTGGGCGA
- TCGAGTGACAATCACTTGTCGGGCTAGTCAGGGCATTTCTAGCAACATTG
- TGTGGCTCCAGCAGAAACCTGGCAAAGCCCCAAAAGGCCTCATCTACCAC
- GGAACCAACCTGGAATCTGGCGTGCCATCTCGGTTTAGTGGATCTGGATC
- CGGGACCGATTACACACTCACCATCTCTTCACTGGAACCTGAGGATTTCG
- CCACCTACTACTGTGTCCAGTACTCCCAGTTTCCACCCACTTTTGGACAG
- GGAACCAAACTCGAGATCAAA;
- d. a light chain variable region comprising the amino acid sequence:
- (SEQ ID NO:
- 4)
- DIQLTQSPSSLSASVGDRVTITCRASQGISSNIVWLQQKPGKAPKGLIYH
- GTNLESGVPSRFSGSGSGTDYTLTISSLEPEDFATYYCVQYSQFPPTFGQ
- GTKLEIK;
- wherein said antibody binds human immunoglobulin heavy chain variable region germline gene VH1-69.
31 more elements
An isolated humanized monoclonal antibody, wherein said antibody comprises:
- a. a heavy chain variable region encoded by the nucleic acid sequence comprising:
- (SEQ ID NO:
- 11)
- CAGGTCCAGCTCGTCCAGTCCGGCGCTGAAGTGGTGAAACCCGGGGCATC
- CGTCAAAGTCTCTTGTAAGGCTAGTGGCTACACCTTCACATCCTACTGGA
- TGCATTGGGTGAAACAGGCACCTGGCCAGGGACTCGAATGGATCGGAGCC
- GTGTCTCCTGGAAATTCCGACACCTCCTACAACGAAAAATTCAAGGGCAA
- GGCAACCCTCACTGTGGACAAATCTGCCTCTACCGCCTACATGGAACTCT
- CATCTCTCCGCTCTGAGGATACTGCTGTGTACTACTGTACCCGGTCACGA
- TACGGCAATAACGCCCTCGATTACTGGGGGCAGGGAACTCTGGTCACTGT
- GTCT;
- b. a heavy chain variable region comprising the amino acid sequence:
- (SEQ ID NO:
- 12)
- QVQLVQSGAEVVKPGASVKVSCKASGYTFTSYWMHWVKQAPGQGLEWIGA
- VSPGNSDTSYNEKFKGKATLTVDKSASTAYMELSSLRSEDTAVYYCTRSR
- YGNNALDYWGQGTLVTVS;
- c. a light chain variable region encoded by the nucleic acid sequence comprising:
- (SEQ ID NO:
- 3)
- GATATTCAGCTCACACAGAGCCCATCTTCTCTGTCTGCTTCTGTGGGCGA
- TCGAGTGACAATCACTTGTCGGGCTAGTCAGGGCATTTCTAGCAACATTG
- TGTGGCTCCAGCAGAAACCTGGCAAAGCCCCAAAAGGCCTCATCTACCAC
- GGAACCAACCTGGAATCTGGCGTGCCATCTCGGTTTAGTGGATCTGGATC
- CGGGACCGATTACACACTCACCATCTCTTCACTGGAACCTGAGGATTTCG
- CCACCTACTACTGTGTCCAGTACTCCCAGTTTCCACCCACTTTTGGACAG
- GGAACCAAACTCGAGATCAAA;
- d. a light chain variable region comprising the amino acid sequence:
- (SEQ ID NO:
- 4)
- DIQLTQSPSSLSASVGDRVTITCRASQGISSNIVWLQQKPGKAPKGLIYH
- GTNLESGVPSRFSGSGSGTDYTLTISSLEPEDFATYYCVQYSQFPPTFGQ
- GTKLEIK;
- or
- e. any of the following:
- (a), (b), (c), or (d);
- wherein said antibody binds human immunoglobulin heavy chain variable region germline gene VH1-69.
34 more elements
Dependencies are inferred from the claim text.