Method and kit for quantifying cardiolipin
AssigneeShiga University of Medical Science
InventorShin-ya Morita
Life of this patent
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Abstract
A method for quantifying cardiolipin in a sample, comprises the steps of: (1) treating the sample with phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand; and a kit for quantifying cardiolipin comprises phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase.
Claims7
A method for quantifying cardiolipin in a sample, comprising the steps of:
- (1) treating the sample in a container with biologically pure phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and
- (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand.
A kit for quantifying cardiolipin comprising:
- (a) a container;
- and
- (b) biologically pure phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase.
Dependencies are inferred from the claim text.