Genetically modified microorganism for producing medium-chain lauric acid and/or dodecanedioic acid and method of using thereof
AssigneeIndustrial Technology Research Institute
InventorsHsin-Ju Hsieh, Yu-Ju Lin, Hwei-Jiung Wang, Chwan-Deng Hsiao, Cheng-Chung Lee, Ya-Wen Chen
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Abstract
Described herein are genetically-modified microorganisms for producing medium chain lauric acid and/or dodecanedioic acid and methods of using the microorganisms. The microorganisms contain a nucleic acid encoding an Umbellularia californica lauroyl ACP-thioesterase (BTE) operably linked to a promoter.
Claims10
A genetically modified microorganism, comprising a nucleic acid encoding an Umbellularia californica lauroyl acyl carrier protein (ACP)-thioesterase (BTE) operably linked to a promoter, wherein the microorganism produces an increased amount of medium-chain lauric acid and/or dodecanedioic acid as compared to the unmodified parent of the microorganism, wherein the Umbellularia californica lauroyl ACP-thioesterase (BTE) is a modified Umbellularia californica lauroyl ACP-thioesterase (BTE), and the modified Umbellularia californica lauroyl ACP-thioesterase (BTE) consists of residues 17-283 of a wild-type Umbellularia californica lauroyl ACP-thioesterase (BTE) or consists of residues 17-283 of a wild-type Umbellularia californica lauroyl ACP-thioesterase (BTE) and a C-terminal His-tag fusion, wherein the amino acid sequence of the wild-type Umbellularia californica is the sequence of SEQ ID NO:
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Dependencies are inferred from the claim text.