Bacteria engineered for ester production
AssigneeThe Regents of the University of California
InventorsShota Atsumi, Gabriel Rodriguez, Yohei Tashiro
Life of this patent
Estimating the term…
Abstract
The present disclosure provides recombinant bacteria with elevated 2-keto acid decarboxylase and alcohol transferase activities. Some recombinant bacteria further have elevated aldehyde dehydrogenase activity. Some recombinant bacteria further have reduced alcohol dehydrogenase and/or aldehyde reductase activity. Methods for the production of the recombinant bacteria, as well as for use thereof for production of various esters are also provided.
Claims24
An Escherichia coli bacterium comprising at least one recombinant polynucleotide encoding a plurality of enzymes, wherein the plurality of enzymes comprise an acetolactate synthase (ALS), an acetohydroxy acid isomeroreductase (AHIR) and a dihydroxy acid dehydratase (DHAD), either a 2-keto acid decarboxylase (KDC) or a 2-ketoisovalerate dehydrogenase enzyme complex (KIVDH), an alcohol dehydrogenase (ADH), an alcohol transferase (ATF), an acetate kinase A (AckA), and a phosphate acetyltransferase (Pta), and wherein expression of the plurality of enzymes permits the bacterium to grow in culture medium comprising acetate and glucose, and results in an increase in production of an ester as compared to a corresponding bacterium lacking the at least one recombinant polynucleotide.
Dependencies are inferred from the claim text.