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US 10,174,330 B2Granted
Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
AssigneeInvista North America S.A.R.L.
InventorsAlex Van Eck Conradie, Adriana Leonora Botes
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Abstract
This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6; backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.
Claims28
A recombinant host comprising
- (1) one or more exogenous nucleic acids encoding:
- (i) a polypeptide having the activity of an acetoacetyl-CoA synthase classified under EC 2.3.1.194;
- (ii) a polypeptide having the activity of a 3-hydroxyacyl-CoA dehydrogenase classified under EC 1.1.1.35 or EC 1.1.1.157, or a 3-oxoacyl-CoA reductase classified under EC 1.1.1.100 or EC 1.1.1.36;
- (iii) a polypeptide having the activity of an enoyl-CoA hydratase classified under EC 4.2.1.17 or EC 4.2.1.119;
- and (iv) a polypeptide having the activity of a trans-2-enoyl-CoA reductase classified under EC 1.3.1.38, EC 1.3.1.8, or EC 1.3.1.44, and
- (2) one or more exogenous nucleic acids encoding either (i) a polypeptide having the activity of a thioesterase classified under EC 3.1.2.-, or (ii) a polypeptide having the activity of an aldehyde dehydrogenase classified under EC 1.2.1.4 and a polypeptide having the activity of a butanal dehydrogenase classified under EC 1.2.1.57,
- wherein the recombinant host produces hexanoate.
4 more elements
Dependencies are inferred from the claim text.