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US 10,173,357 B2Granted
Poly(ethylene glycol) cross-linking of soft materials to tailor viscoelastic properties for bioprinting
AssigneeNorthwestern University
InventorsAlexandra L. Rutz, Ramille N. Shah
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Abstract
Extrudable hydrogel compositions for printing 3D objects, such as cell growth scaffolds, are provided. Also provided are methods for making the crosslinked hydrogel compositions and the printed objects and methods for culturing cells using the cell growth scaffolds. The hydrogel precursor solutions are aqueous solutions comprising a biocompatible polymer, functionalized polyethylene glycol as a crosslinker and, optionally, cells and/or bioactive factors.
Claims20
A method of forming a three-dimensional object, the method comprising:
- extruding a hydrogel composition having a shear storage modulus of less than 300 Pa through the annulus of a channel, the hydrogel composition comprising:
- water;
- a crosslinked biocompatible polymer;
- and a bioactive factor, cells or a combination thereof, wherein the crosslinks between the biocompatible polymer chains comprise repeating units having the following structure:
- to form a strand comprising a continuous matrix of the biocompatible polymer, wherein the strand substantially retains the three-dimensional shape imparted to it by the extrusion.
2 more elements
Dependencies are inferred from the claim text.