Modified chymosin polypeptides
AssigneeDsm IP Assets B.V.
InventorsPetrus Jacobus Theodorus Dekker, Rene Marcel De Jong, Michael Dennis Tabeling, Cornelis Marinus Muijlwijk
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Abstract
The invention relates to a polypeptide having chymosin activity which: a. is capable of hydrolyzing bovine alpha s1-casein at position F23F24 so as to form αs1-I CN (f24-199) more rapidly than camel chymosin; and b. has a C/P ratio higher than the C/P ratio of bovine chymosin.
Claims16
A polypeptide having chymosin activity and comprising an amino acid sequence that has at least 90% sequence identity to SEQ ID NO:4, comprises at least one substitution modification relative to SEQ ID NO:4 selected from the group consisting of Q288H, and Q288R, and is capable of hydrolysing bovine alpha s1-casein at position F23F24 so as to form αs1-I CN (f24-199) more rapidly than the polypeptide of SEQ ID NO:4.
Dependencies are inferred from the claim text.