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US 10,167,452 B2Granted
Viral purification methods
AssigneeOncolytics Biotech Inc.
InventorMatthew C. Coffey
Life of this patent
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Abstract
The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography. Viruses and compositions comprising the viruses prepared according to the present invention are also provided.
Claims16
A method of producing reovirus from a culture of cells, comprising the steps of:
- (a) providing a culture of cells which has been infected by the reovirus in culture;
- (b) extracting the reovirus from the culture of cells by adding a detergent to the culture of cells and incubating for a period of time to result in a cell lysate;
- (c) separating cell debris from the reovirus in the cell lysate;
- and
- (d) collecting the reovirus,
- provided that after infection by the reovirus and before extraction of the reovirus, the cells are not pelleted or resuspended.
3 more elements
Dependencies are inferred from the claim text.