Gas evacuation system for nanofluidic biosensor
AssigneeAbionic SA
InventorsNicolas Durand, Iwan Maerki, Matthias Geissbuehler
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Abstract
A nanofluidic biosensor system (200) comprising a bottom substrate (120) and a top substrate (110) between which are defined an input lateral aperture (210), a nanoslit (230) which contains at least one functionalized area (231) and an output lateral aperture (220) or an internal reservoir (221), said biosensor system (200) being adapted to let a solution containing biomolecules (320) enter the input lateral aperture (210) and successively pass through said nanoslit (230) and said output lateral aperture (220) or internal reservoir (221); said biosensor system (200) furthermore comprising a gas evacuation subsystem (150-155) which is located between said nanoslit (230) and the biosensor external environment.
Claims12
A nanofluidic biosensor system comprising:
- a bottom substrate and a top substrate;
- arranged along a flow direction of the biosensor system between the bottom substrate and the top substrate, (i) an input lateral aperture, (ii) a nanoslit having a functionalized area, and then (iii) at least one of an output lateral aperture and an internal reservoir;
- and
- a gas evacuation subsystem located at at least one of the output lateral aperture and the internal reservoir, along the flow direction located between the nanoslit and an external environment of the biosensor downstream of the nanoslit,
- wherein the gas evacuation subsystem evacuates trapped air from the nanofluidic biosensor system.
The method for detecting biomolecules with a nanofluidic system, the nanofluidic system comprising,
- a bottom substrate and a top substrate, an input lateral aperture, a nanoslit having a functionalized area, and at least one of an output lateral aperture and an internal reservoir being arranged between the bottom substrate and the top substrate;
- a gas evacuation subsystem located between the nanoslit and an external environment of the biosensor downstream from the nanoslit;
- and
- an optical system,
- wherein the method comprises the steps of:
- entering a solution having the biomolecules through the input lateral aperture and successively passing the solution through the nanoslit and the at least one of the output lateral aperture and internal reservoir;
- evacuating trapped air bubbles by the gas evacuation system;
- and
- detecting specific biomolecules immobilized on biomarkers inside the nanoslit by quantifying fluorophores attached to the biomolecules of the solution.
Dependencies are inferred from the claim text.