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US 10,161,001 B2Granted
Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
AssigneeThe Trustees of Princeton University
InventorsRobert H. Austin, Zhaoning Yu, Jonas O. Tegenfeldt, Stephen Y. Chou, Han Cao
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Abstract
Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.
Claims16
A nanofluidic chip, comprising:
- a) nanochannel array, comprising:
- a substrate having a surface;
- at least one channel in the surface, wherein the at least one channel has a trench width of less than 100 nanometers and a trench depth of less than 100 nanometers;
- and
- the at least one channel is capable of admitting a fluid;
- b) at least one sample reservoir in fluid communication with the at least one channel, said sample reservoir capable of releasing a fluid;
- and
- c) at least one waste reservoir in fluid communication with the at least one channel, said waste reservoir capable of receiving a fluid.
5 more elements
A system, comprising:
- A) a nanofluidic chip, comprising:
- a) nanochannel array, comprising:
- a substrate having a surface;
- and
- at least one channel in the surface capable of admitting a fluid, wherein the at least one channel has a trench width of less than 100 nanometers and a trench depth of less than 100 nanometers;
- and
- b) at least one sample reservoir in fluid communication with the at least one channel, said sample reservoir capable of releasing a fluid;
- and
- B) an apparatus for detecting at least one signal transmitted from the at least one fluid in the nanochannel array.
6 more elements
A method of analyzing at least one macromolecule, comprising the steps of:
- providing a nanofluidic chip, comprising:
- a) nanochannel array, comprising:
- a substrate having a surface;
- at least one nanochannel in the surface, wherein the at least one channel has a trench width of less than 100 nanometers and a trench depth of less than 100 nanometers;
- and
- the at least one channel is capable of admitting a fluid;
- b) at least one sample reservoir in fluid communication with the at least one channel, said sample reservoir capable of releasing a fluid;
- and
- c) at least one waste reservoir in fluid communication with the at least one channel, said waste reservoir capable of receiving a fluid;
- transporting at least one macromolecule in a fluid sample into the at least one nanochannel, such that the at least one macromolecule is in an elongated state within the at least one nanochannel;
- maintaining the at least one macromolecule in an elongated state within a region of the at least one nanochannel having a substantially constant width;
- detecting at least one signal from the at least one elongated macromolecule within the at least one nanochannel;
- and
- correlating the detected signal to at least one property of the at least one macromolecule.
11 more elements
Dependencies are inferred from the claim text.