Assay for the comprehensive identification of antibiotic sensitivity
AssigneeCap Diagnostics, LLC
InventorsDavid A. Baunoch, Miguel F. R. Penaranda, Michael L. Opel, Maher Badir
Life of this patent
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Abstract
Disclosed herein are methods determining susceptibility of bacteria in a sample from a subject suspected of having an infection to a plurality of antibiotics simultaneously, wherein the sample is tested without first isolating the bacteria from the sample.
Claims25
A method for simultaneously determining the antibiotic susceptibilities of a mixed population of bacteria in a sample from a subject suspected of having an infection, the method comprising:
- (a) applying portions of the sample simultaneously to a plurality of antibiotic-containing growth media, wherein, in a first subset of the antibiotic-containing growth media, each antibiotic-containing growth medium contains a combination of antibiotics and, in a second subset of the antibiotic-containing growth media, each antibiotic-containing growth medium contains one antibiotic, and wherein the bacteria are not isolated from the sample prior to applying to the antibiotic-containing growth media;
- (b) culturing the sample portions on or in the antibiotic-containing growth media for a period of time;
- (c) suspending the bacteria present in each antibiotic-containing medium with a solution and recovering the suspensions;
- (d) measuring an OD600 of each suspension, and calculating an adjusted OD600 for each suspension, wherein the adjusted OD600 of each suspension indicates the susceptibility or resistance of the bacteria from the sample to each combination of antibiotics and to each antibiotic tested alone;
- and
- (e) providing the susceptibility information to a medical professional to determine antibiotic therapy for the subject.
A method for simultaneously determining the antibiotic susceptibilities of a mixed population of bacteria in a sample, the method comprising:
- (a) mixing a quantity of a sample with a growth medium and incubating for a period of time, wherein the sample is from a subject suspected of having an infection;
- (b) applying portions of the incubated sample simultaneously to a plurality of antibiotic-containing growth media, wherein, in a first subset of the antibiotic-containing growth media, each antibiotic-containing growth medium contains a combination of antibiotics and;
- in a second subset of the antibiotic-containing growth media, each antibiotic-containing growth medium contains one antibiotic;
- wherein the bacteria are not isolated from the sample prior to applying to the antibiotic-containing growth media;
- (c) culturing the sample portions in the antibiotic-containing growth media for a period of time;
- (d) measuring bacterial growth in the antibiotic-containing growth media by measuring the OD600, and calculating an adjusted OD600 for each antibiotic-containing growth medium, wherein the adjusted OD600 measurements of the antibiotic-containing growth media indicate the susceptibility or resistance of the bacteria, from the sample to each combination of antibiotics and to each antibiotic tested alone;
- and
- (e) providing the susceptibility information to a medical professional to determine antibiotic therapy for the subject.
Dependencies are inferred from the claim text.