Device for lysing biological species and method implemented by said device

A method for the mechanical lysis of a biological species comprising the following steps:

• A) Providing a device for the mechanical lysis of a biological species contained in a fluid, comprising a first wall and a second wall mounted movable relative to one another between an initial position in which the first and second walls are drawn apart and a lysis position in which the first wall presses against the second wall, the first and second walls also being mounted movable under shear relative to one another in the lysis position, wherein at least one of the first and second walls has a rough bearing surface against the other wall and has an average surface roughness parameter Ra of between 0.2 μm and 10 μm, or between 0.2 μm and 3 μm so that the first and second walls lie in the initial position;
• B) Placing the biological species to be lysed between the first and second walls;
• C) Bringing the first and second walls to the lysis position;
• D) Generating shear between the first and second walls in the lysis position to cause mechanical lysis of the biological species and to obtain a lysate;
• E) Drawing the first and second walls apart until they reach the initial position;
• and
• F) Recovering the lysate of the biological species.