Transposition of native chromatin for personal epigenomics
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsPaul Giresi, Jason D. Buenrostro, Howard Y. Chang, William J. Greenleaf
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Abstract
Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided.
Claims23
A method for analyzing a biological sample, comprising:
- (a) contacting chromatin of a genome region of said biological sample with an insertional enzyme complex to produce tagged nucleic acid molecules, wherein said insertional enzyme complex does not comprise an antibody specific to a protein that is part of said chromatin;
- and
- (b) performing a nucleic acid assay on said tagged nucleic acid molecules or derivatives thereof, to provide sequence information of said tagged nucleic acid molecules or derivatives thereof.
Dependencies are inferred from the claim text.