Back to results
US 10,149,863 B2Granted
NKT-like cell subpopulation and method of using the same in the treatment of tumor
AssigneeTsinghua University
InventorMinghui Zhang
Life of this patent
Estimating the term…
Abstract
The disclosure discloses a kind of new NKT-like cell subpopulation, a therapeutical composition comprising the NKT-like cell subpopulation, and the medical use thereof. The disclosure also provides a preparation method of the NKT-like cell subpopulation. The disclosed NKT-like cell subpopulation has a strong antitumor effect, and can be adoptive transferred into a subject to treat the tumor in the subject after in vitro cultured and amplified.
Claims14
A preparation method of isolated mammal NKT-like cells, comprising the following steps:
- 1) collecting peripheral blood from a mammal subject, separating and removing red blood cells from the peripheral blood;
- 2) isolating mononuclear cells from the peripheral blood obtained in step 1) from which red blood cells have been removed, and sorting the NKT-like cells by using a cell sorting technique with NKT-like cell surface markers;
- 3) in vitro culturing the NKT-like cells obtained in step 2), and adding cytokine(s) that stimulate the proliferation and activation of T cell, for a period of time enough to amplify the number of the NKT-like cells by at least 10˜1000 times;
- and
- 4) harvesting the NKT-like cells obtained in step 3),
- wherein the NKT-like cell surface markers comprise CD8, CD3, TCRαβ, CD161c, CD44, CD62L, CD122, NKG2A/C/E, KLRG1, NKG2D, Ly49G2 and CD27, or comprise CD8, CD3, TCRαβ, CD56, CD44, CD62L, CD122, NKG2A/C/E, KLRG1, NKG2D, Ly49G2 and CD27.
3 more elements
A preparation method of isolated mammal NKT-like cells, comprising the following steps:
- 1) collecting peripheral blood from a mammal subject, separating and removing red blood cells from the peripheral blood;
- 2) isolating mononuclear cells from the peripheral blood obtained in step 1) from which red blood cells have been removed, in vitro culturing the isolated mononuclear cells, and adding culture cytokine(s) that stimulate the proliferation and activation of T cell, for a period of time enough to amplify the number of the mononuclear cells by at least 10˜1000 times;
- 3) sorting the NKT-like cells by using a cell sorting technique with NKT-like cell surface markers;
- and
- 4) harvesting the NKT-like cells obtained in step 3),
- wherein the NKT-like cell surface markers comprise CD8, CD3, TCRαβ, CD161c, CD44, CD62L, CD122, NKG2A/C/E, KLRG1, NKG2D, Ly49G2 and CD27, or comprise CD8, CD3, TCRαβ, CD56, CD44, CD62L, CD122, NKG2A/C/E, KLRG1, NKG2D, Ly49G2 and CD27.
3 more elements
Dependencies are inferred from the claim text.