Inactivating pathogens with oxidizing agents for vaccine production
AssigneeOregon Health & Science University
InventorsMark K. Slifka, Shirley Villadiego, Erika Hammarlund, Paul Yoshihara
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Abstract
The present disclosure provides methods for producing a vaccine composition containing a pathogen that is rendered noninfectious by exposure to hydrogen peroxide. The methods disclosed herein are suitable for the preparation of vaccines for a wide variety of pathogens, including viruses, bacteria and parasites. The disclosure also provides vaccine compositions (medicaments) containing a pathogen inactivated by exposure to hydrogen peroxide. Methods for eliciting an immune response in a subject by administering vaccine compositions containing a hydrogen peroxide inactivated pathogen are also provided.
Claims31
An immunogenic vaccine composition having an oxidizing agent-inactivated viral pathogen, produced by a method comprising:
- contacting a cultured, isolated, or purified viral pathogen with a solution comprising a chemical oxidizing agent in an amount and for a time-period sufficient for the chemical oxidizing agent to render the viral pathogen noninfectious while retaining viral immunogenicity, wherein the chemical oxidizing agent comprises one or more of the oxidizing agents selected from the group consisting of Sn4+, Cu2+, ClO4−, AgCl, ClO3−, Cu+, I2, ClO2−, Fe3+, Hg22+, Ag+, Hg2+, ClO−, 2Hg2+, NO3−, Br2, O2, Cr2O72−, Cl2, Ce4+, MnO4−, H2O2, Co3+, S2O82−, and F2, or a salt thereof, but not including O3, or hypoiodite (IO−) or a salt thereof;
- and
- verifying immunogenicity of the noninfectious virus by using it to elicit a virus-specific antibody, B cell or T cell response, or to reduce infection by the viral pathogen, or decrease symptoms that result from infection by the viral pathogen, wherein an immunogenic vaccine composition comprising an inactivated viral pathogen is provided.
An immunogenic vaccine composition comprising:
- a chemically-oxidized inactivated cultured, isolated, or purified viral pathogen, which inactivated viral pathogen retains one or more predominant antigenic epitopes of the biologically active viral pathogen suitable to elicit a virus-specific antibody, B cell or T cell response, or to reduce infection by the viral pathogen, or decrease symptoms that result from infection by the viral pathogen, wherein the chemical oxidizing agent comprises one or more of the oxidizing agents selected from the group consisting of Sn4+, Cu2+, ClO4−, AgCl, ClO3−, Cu+, I2, ClO2−, Fe3+, Hg22+, Ag+, Hg2+, ClO−, 2Hg2+, NO3−, Br2, O2, Cr2O72−, Cl2, Ce4+, MnO4−, H2O2, Co3+, S2O82−, and F2, or a salt thereof, but not including O3 or hypoiodite (IO−) or a salt thereof.
Dependencies are inferred from the claim text.