Integrated heater and magnetic separator
AssigneeHandyLab, Inc.
InventorKalyan Handique
Life of this patent
Estimating the term…
Abstract
An apparatus for providing thermal and magnetic energy to a receptacle containing a reaction mixture and a magnetic retention member. The apparatus can also control heating of a reaction mixture, and bring about a separation of magnetic particles from the reaction mixture. The reaction mixture typically comprises polynucleotides from a biological sample that are being brought into a PCR-ready form.
Claims18
A method of extracting nucleic acids using a rack and an integrated separator and heater, the integrated separator and heater comprising a plurality of heater units aligned collinearly along a horizontal first axis, the integrated separator and heater further comprising one or more magnets disposed on a first side of the plurality of heater units and aligned along a second axis parallel to the first axis, the method comprising:
- inserting a plurality of holders in the rack, each holder comprising a single process tube of a plurality of process tubes;
- inserting the rack into the integrated separator and heater such that at least a portion of rack is removably received on a second side of the plurality of heater units opposite the one or more magnets on the first side, wherein a portion of each process tube of the plurality of process tubes is received in a single heater unit of the plurality of heater units when the rack is inserted into the integrated separator and heater;
- moving all of the one or more magnets backwards and forwards together in a vertical direction perpendicular to the second axis while maintaining proximity to an exterior side of each of the process tubes received in the integrated separator and heater;
- during at least a portion of the motion, moving magnetic particles contained in the process tubes;
- and
- independently controlling each heater unit of the plurality of heater units to apply heat to the plurality of process tubes received in the integrated separator and heater.
Dependencies are inferred from the claim text.