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US 10,138,494 B2Granted
Whole expressed cell as antigen carrier, and use thereof in preparing vaccine or diagnostic agent, and screening monoclonal antibodies
AssigneeFormosa Biomedical Inc.
InventorShun-Wen Ko
Life of this patent
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Abstract
A mammalian cell co-transfect with an expression plasmid comprising T7 promoter and an open reading frame (ORF) of target antigen, and a vT7 recombinant vaccinia virus expressing T7 polymerase. The entire antigen expressing cell is used as a carrier of the target antigen for preparing a vaccine or diagnostic agent, and screening monoclonal antibodies.
Claims8
An animal cell expression system for a target antigen of porcine epidemic diarrhea virus (PEDV), comprising an animal cell co-transfected with:
- (a) an expression plasmid containing a T7 promoter and an open reading frame (ORF) for a FEDV viral antigen protein or its fragment;
- and
- (b) a vT7 recombinant vaccinia virus (vT7FBI) expressing T7 polymerase comprising a coding nucleotide sequence of SEQ ID No.3.
Dependencies are inferred from the claim text.