Enzymatically produced cellulose
AssigneeE. I. Du Pont de Nemours and Company
InventorsNatnael Behabtu, Ayrookaran J. Poulose, Zheyong Yu, Zhenghong Zhang
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Abstract
Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
Claims27
An isolated reaction composition comprising water, glucose-1-phosphate, cellodextrin, and a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, wherein the cellodextrin phosphorylase enzyme synthesizes insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15.
A method for producing insoluble cellulose, said method comprising:
- a) contacting at least water, glucose-1-phosphate, cellodextrin, and a cellodextrin phosphorylase enzyme in an isolated reaction, wherein said cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6,
- wherein insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15 is produced;
- and
- b) optionally, isolating the insoluble cellulose produced in step (a).
Dependencies are inferred from the claim text.