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AssigneeCentro de Ingenieria Genetica y Biotecnologia
InventorsEnrique Rosendo Pérez Cruz, Lázaro Hernández García, Duniesky Martínez García, Luis Enrique Trujillo Toledo, Carmen Menéndez Rodríguez, Alina Sobrino Legón, Ricardo Ramírez Ibañez, Gumersindo Feijoo Costa, Juan Manuel Lema Rodicio
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The present invention discloses an industrial scale method to obtain 1-kestose by the use of a recombinant fructosyltransferase (FTF), isolated from Festuca arundinacea, expressed constitutively in a non-saccharolytic yeast. In this invention, the recombinant FTF type sucrose:sucrose 1-fructosyltransferase (1-SSTrec) is produced constitutively, stable and at high yield, both in the culture supernatant and in intact cells of the host Pichia pastoris. Hence, the invention additionally provides a method for 1-SST production at industrial scale. The recombinant enzyme is then used for mass production of short-chain fructooligosaccharides (FOS), specifically 1-kestose, from sucrose. The method of the present invention establishes conditions that allow conversion rates where the synthesized FOS constitute above 55% (w/w) of the total sugars in the reaction mixture and the 1-kestose content reaches values higher than 90% (w/w) of the total FOS fraction.
A method for the production of sucrose:sucrose 1-fructosyltransferase (1-SST) on an industrial scale comprising the fermentation of a non-saccharolytic yeast that expresses constitutively at least nine copies of the gene encoding 1-SST, isolated from Festuca arundinacea integrated in the genome, wherein expression of the gene is under transcriptional control by a yeast constitutive promoter, wherein carbon source is glycerol, glucose and/or sucrose of any degree of purity-.
Dependencies are inferred from the claim text.