Process for producing an organic compound and an intermediary compound
AssigneePhotanol B.V.
InventorsKlaas Jan Hellingwerf, Maarten Joost Teixeira de Mattos
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Abstract
A process of producing an organic compound and/or an intermediary compound by feeding carbon dioxide to a culture of Cyanobacteria cells and subjecting the culture to light, wherein the cells are capable of expressing a nucleic acid molecule that confers the ability to convert a glycolytic intermediate into said organic/intermediary compound. The expression of the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in the culture.
Claims15
A process of producing ethylene and succinate, comprising feeding carbon dioxide to a culture of cyanobacterial cells and subjecting said culture to light,
- wherein said cyanobacterial cells express a nucleic acid molecule encoding at least one enzyme that confers on the cells an ability to convert a glycolytic intermediate into ethylene and succinate,
- wherein said nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in said culture, and
- wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:
- 92 or the nucleic acid molecule encodes for at least one enzyme having the amino acid sequence of SEQ ID NO:
- 91.
A Cyanobacterium that expresses a nucleic acid molecule encoding at least one enzyme that confers on the Cyanobacterium an ability to convert a glycolytic intermediate into ethylene and succinate,
- wherein the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient when culturing said Cyanobacterium, and
- wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:
- 92 or the nucleic acid molecule encodes for at least one enzyme having the amino acid sequence of SEQ ID NO:
- 91.
Dependencies are inferred from the claim text.