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US 10,131,882 B2Granted
Methods and products for transfection
AssigneeFactor Bioscience Inc.
InventorsMatthew Angel, Christopher Rohde
Life of this patent
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Abstract
The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed.
Claims16
A method for reprogramming a differentiated cell to a less differentiated state, comprising:
- (a) providing a differentiated cell;
- (b) culturing the differentiated cell;
- (c) transfecting the differentiated cell with one or more synthetic RNA molecules, wherein the one or more synthetic RNA molecules include at least one RNA molecule encoding one or more reprogramming factors and wherein the transfecting results in the cell expressing the one or more reprogramming factors;
- and
- (d) repeating step (c) at least twice during 5 consecutive days, wherein the amount of one or more synthetic RNA molecules transfected in one or more later transfections is greater than the amount transfected in one or more earlier transfections, to result in the cell being reprogrammed to a less differentiated state,
- wherein steps (c) and (d) occur in the presence of a medium containing ingredients that support reprogramming of the differentiated cell to a less differentiated state.
3 more elements
A method for reprogramming a non-pluripotent cell, comprising:
- (a) providing a non-pluripotent cell;
- (b) culturing the non-pluripotent cell;
- (c) transfecting the non-pluripotent cell with one or more synthetic RNA molecules, wherein the one or more synthetic RNA molecules include at least one RNA molecule encoding one or more reprogramming factors and wherein the transfecting results in the cell expressing the one or more reprogramming factors;
- and
- (d) repeating step (c) at least twice during 5 consecutive days, wherein the amount of one or more synthetic RNA molecules transfected in one or more later transfections is greater than the amount transfected in one or more earlier transfections, to result in the non-pluripotent cell being reprogrammed,
- wherein steps (c) and (d) occur in the presence of a medium containing ingredients that support reprogramming of the non-pluripotent cell.
3 more elements
Dependencies are inferred from the claim text.