Methods for assessing biospecimen integrity
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsChad Borges, Joshua Jeffs
Life of this patent
Estimating the term…
Abstract
Methods for quantifying biospecimen sample integrity using oxidation. Embodiments include assessing ex vivo oxidation of a biospecimen sample by quantifying a difference in protein oxidation of the biospecimen sample in comparison to protein oxidation of a portion of the biospecimen sample intentionally incubated to cause oxidation to hit its maximum value. The difference in a level of oxidation in the biospecimen sample and the portion of the biospecimen sample is then inversely proportional to the degree of ex vivo oxidation.
Claims8
A method for assessing ex vivo oxidation of a stored biospecimen sample, comprising:
- measuring protein oxidation of at least one of an albumin and apolipoprotein A-1 (apoA-1) in said stored biospecimen sample;
- measuring protein oxidation of at least one of an albumin and apolipoprotein A-1 (apoA-1) in a portion of said stored biospecimen sample intentionally incubated to cause oxidation to hit its maximum value;
- quantifying a difference in protein oxidation of said at least one of an albumin and apolipoprotein A-1 (apoA-1) of said stored biospecimen sample in comparison to protein oxidation of at least one of an albumin and apolipoprotein A-1 (apoA-1) in said portion of said stored biospecimen sample intentionally incubated to cause oxidation to hit its maximum value, wherein said difference in a level of oxidation in the stored biospecimen sample and said portion of the stored biospecimen sample is inversely proportional to a degree of ex vivo oxidation.
A method for assessing oxidation of a stored biospecimen sample comprising:
- quantifying an amount of protein oxidation of at least one of an albumin and apolipoprotein A-1 (apoA-1) in said stored biospecimen sample;
- quantifying an amount of protein oxidation of at least one of an albumin and apolipoprotein A-1 (apoA-1) in a portion of said stored biospecimen sample intentionally incubated to cause oxidation to hit its maximum value;
- and
- comparing said amount of protein oxidation of at least one of an albumin and apolipoprotein A-1 (apoA-1) in said stored biospecimen sample to that of the portion of said stored biospecimen sample intentionally incubated to cause oxidation to hit its maximum value, wherein a difference in a level of oxidation in the stored biospecimen sample and said portion of the stored biospecimen sample is inversely proportional to a degree of oxidation.
Dependencies are inferred from the claim text.