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AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsXuan Wang, Reed Cartwright, Christian Sievert, Taylor Loeffler, Lizbeth Nieves, Larry Panyon, Chandler Morris
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The present invention relates to increasing xylose utilization in industrial microbe by inducing mutations in the regulator genes, crp and xylR. Thus the invention is directed to isolated nucleic acid sequences that encode mutations in the crp gene and the xylR gene and recombinant bacterium that express mutated CRP and XylR. In some embodiments, the mutation results in a point mutation at residue 142 of the CRP protein and/or at point mutation at residues 121, 182 and/or 363 of the XylR protein (based on the protein sequences in E. coli). The invention also includes methods of using the recombinant bacterium.
A method for increasing xylose utilization in microbes of the Enterobacteriaceae family, the method comprising mutating the microbial genomes of the microbes to produce a mutated microbe of the Enterobacteriaceae family having a mutated XylR protein,
A method for improved chemical production from woody biomass comprising culturing a recombinant bacterium having increased xylose utilization with woody biomass, wherein the recombinant bacterium having increased xylose utilization expresses a mutated XylR protein;
Dependencies are inferred from the claim text.