Solid phase peptide synthesis
AssigneeCEM Corporation
InventorJonathan M. Collins
Life of this patent
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Abstract
An improved method of deprotection in solid phase peptide synthesis is disclosed. In particular the deprotecting composition is added in high concentration and small volume to the mixture of the coupling solution, the growing peptide chain, and any excess activated acid from the preceding coupling cycle, and without any draining step between the coupling step of the previous cycle and the addition of the deprotection composition for the successive cycle. Thereafter, the ambient pressure in the vessel is reduced with a vacuum pull to remove the deprotecting composition without any draining step and without otherwise adversely affecting the remaining materials in the vessel or causing problems in subsequent steps in the SPPS cycle.
Claims14
A method of deprotection in solid phase peptide synthesis in which the improvement comprises:
- adding an organic base deprotection composition neat to a mixture of a growing peptide chain, a solid phase support, and any excess activated amino acid from a preceding coupling cycle;
- wherein the organic base is added to the mixture in a volume of 20% or less of the volume of the mixture;
- and
- without any draining step between the coupling step of any preceding coupling cycle and the addition of a deprotection composition for any successive cycle.
A method of deprotection in solid phase peptide synthesis in which the improvement comprises:
- deprotecting a protected amino acid by combining and heating the protected amino acid and a liquid organic base in a reaction vessel;
- and
- during or after the deprotecting step, reducing the ambient pressure in the vessel with a vacuum pull to remove the liquid organic base without any intervening draining step while simultaneously heating the vessel contents;
- and
- without otherwise adversely affecting the remaining materials in the vessel or causing problems in any subsequent steps in the SPPS cycle.
A method of deprotection in solid phase peptide synthesis (SPPS) in which the improvement comprises deprotecting in a reaction vessel a protected amino acid below atmospheric pressure at a temperature of at least about 60° C. while providing a path for evaporating base to leave the reaction vessel.
Dependencies are inferred from the claim text.