# US 10,121,644 B2 · Mass spectrometer and mass spectrometry method

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- Number: US 10,121,644 B2
- Document ID: US10121644B2
- Status: Granted
- Kind: B2
- Application number: 15/110,306
- Filed: 2014-02-10
- Published: 2016-11-10
- Granted: 2018-11-06
- Earliest claimed: 2014-02-10
- Assignees: Shimadzu Corporation
- Applicants: Shimadzu Corporation
- Inventors: Shinjiro Fujita
- Web page: https://patentgrep.com/patent/US10121644B2

## CPC

- H01J 49/165: {Electrospray ionisation}
- G01N 30/8631: {Peaks}
- G01N 30/8651: {Recording, data aquisition, archiving and storage}
- H01J 49/0031: {Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence)}
- H01J 49/107: {Arrangements for using several ion sources}
- H01J 49/168: {field ionisation, e.g. corona discharge (atmospheric pressure corona discharge per se H01T19/00)}
- H01J 49/24: Vacuum systems, e.g. maintaining desired pressures
- H01J 49/4215: {Quadrupole mass filters (H01J49/4225 takes precedence)}
- G01N 30/7266: {by electric field, e.g. electrospray}
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A mass spectrometer including an ionization source including an ESI probe (201), an ESI power source (24), a corona needle (202) and an APCI power source (24); an ionization condition storage section (41) for storing a plurality of ionization conditions related to the liquid sample, set by an analysis operator, with the ionization conditions differing from each other in the value of the ESI voltage or/and the value of the APCI voltage; a mass spectrometry executer (43) for conducting a mass spectrometry for an ion generated from the liquid sample using each of the plurality of ionization conditions; and a mass spectrometry result selector (44) for selecting, for each of the one or plurality of components, a mass spectrometry result in which the ion is detected with a suitable level of intensity for an analysis, from the mass spectrometry results respectively obtained for the plurality of ionization conditions.

## Claims

### Claim 1

A liquid chromatograph mass spectrometer, comprising:
a) an ionization source including: an ESI probe into which a liquid sample containing one or a plurality of components separated from each other in a column of a liquid chromatograph is introduced; a corona needle located near an exit port of the ESI probe; an ESI voltage supplier for supplying the ESI probe with an ESI voltage; and an APCI voltage supplier for supplying the corona needle with an APCI voltage;
b) an ionization condition storage section for storing a plurality of ionization conditions related to the liquid sample, set by an analysis operator, with the ionization conditions differing from each other in a value of the ESI voltage or/and a value of the APCI voltage;
c) a mass spectrometry executer for conducting a mass spectrometry for an ion generated from the one or the plurality of components using the plurality of ionization conditions cyclically and obtaining a plurality of mass chromatograms each of which corresponds to each of the plurality of ionization conditions; and
d) a mass spectrometry result selector for selecting, for each of the one or the plurality of components, a mass spectrometry result in which the ion is detected with a suitable level of intensity for an analysis, from the mass spectrometry results respectively obtained for the plurality of ionization conditions based on the plurality of mass chromatograms.

### Claim 2 (depends on 1)

The liquid chromatograph mass spectrometer according to claim 1, wherein the mass spectrometry result selector selects a mass spectrometry result in which a detection intensity of the ion is at a highest level.

### Claim 3 (depends on 1)

The liquid chromatograph mass spectrometer according to claim 1, wherein the mass spectrometry result selector selects a mass spectrometry result in which a ratio of a detection intensity of the ion to a noise level is at a highest level.

### Claim 4 (depends on 1)

The liquid chromatograph mass spectrometer according to claim 1, comprising:
e) an ionization condition determiner for determining, for each of the one or plurality of components, the ionization condition corresponding to the selected mass spectrometry result as an optimum ionization condition.

### Claim 5 (depends on 1)

The liquid chromatograph mass spectrometer according to claim 1, comprising:
f) a display unit; and
g) a mass spectrometry result displayer for displaying, on the display unit, the mass spectrometry result selected by the mass spectrometry result selector.

### Claim 6

A method for performing a liquid chromatograph mass spectrometry for an ion generated from a liquid sample containing one or a plurality of components separated from each other in a column of a liquid chromatograph in an ionization source provided in a mass spectrometer, the ionization source including an ESI source for ionizing the liquid sample by applying an ESI voltage to an ESI probe into which the liquid sample is introduced and an APCI source for ionizing the liquid sample discharged from the ESI probe by applying an APCI voltage to a corona needle located near an exit port of the ESI probe, the method comprising steps of:
a) allowing an analysis operator to set a plurality of ionization conditions for an ionization of the liquid sample, with the ionization conditions differing from each other in a value of the ESI voltage or/and a value of the APCI voltage;
b) performing a mass spectrometry for an ion generated from the one or the plurality of components using the plurality of ionization condition cyclically and obtaining a plurality of mass chromatograms each of which corresponds to each of the plurality of ionization conditions; and
c) selecting, for each of the one or the plurality of components, a mass spectrometry result in which the ion is detected with a suitable level of intensity for an analysis, from the mass spectrometry results respectively obtained for the plurality of ionization conditions based on the plurality of mass chromatograms.

### Claim 7 (depends on 2)

The liquid chromatograph mass spectrometer according to claim 2, comprising:
e) an ionization condition determiner for determining, for each of the one or plurality of components, the ionization condition corresponding to the selected mass spectrometry result as an optimum ionization condition.

### Claim 8 (depends on 3)

The liquid chromatograph mass spectrometer according to claim 3, comprising:
e) an ionization condition determiner for determining, for each of the one or plurality of components, the ionization condition corresponding to the selected mass spectrometry result as an optimum ionization condition.

### Claim 9 (depends on 2)

The liquid chromatograph mass spectrometer according to claim 2, comprising:
f) a display unit; and
g) a mass spectrometry result displayer for displaying, on the display unit, the mass spectrometry result selected by the mass spectrometry result selector.

### Claim 10 (depends on 3)

The liquid chromatograph mass spectrometer according to claim 3, comprising:
f) a display unit; and
g) a mass spectrometry result displayer for displaying, on the display unit, the mass spectrometry result selected by the mass spectrometry result selector.

### Claim 11 (depends on 4)

The liquid chromatograph mass spectrometer according to claim 4, comprising:
f) a display unit; and
g) a mass spectrometry result displayer for displaying, on the display unit, the mass spectrometry result selected by the mass spectrometry result selector.

### Claim 12 (depends on 7)

The liquid chromatograph mass spectrometer according to claim 7, comprising:
f) a display unit; and
g) a mass spectrometry result displayer for displaying, on the display unit, the mass spectrometry result selected by the mass spectrometry result selector.

### Claim 13 (depends on 8)

The liquid chromatograph mass spectrometer according to claim 8, comprising:
f) a display unit; and
g) a mass spectrometry result displayer for displaying, on the display unit, the mass spectrometry result selected by the mass spectrometry result selector.