# US 10,120,180 B2 · Methods and apparatus for stretched light field microscope > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,120,180 B2 - Document ID: US10120180B2 - Status: Granted - Kind: B2 - Application number: 14/868,340 - Filed: 2015-09-28 - Published: 2016-03-31 - Granted: 2018-11-06 - Earliest claimed: 2014-09-28 - Assignees: Massachusetts Institute of Technology - Applicants: Moshe Ben Ezra; Edward Boyden; Christopher Rowlands; Young Gyu Yoon - Inventors: Moshe Ben Ezra; Edward Boyden; Christopher Rowlands; Young Gyu Yoon - Web page: https://patentgrep.com/patent/US10120180B2 ## CPC - G02B 21/361: {Optical details, e.g. image relay to the camera or image sensor (G02B21/364 takes precedence; illumination details G02B21/06 and subgroups)} - G02B 21/0004: {specially adapted for specific applications} - G02B 21/367: {providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison} - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS ## Abstract The information budget of a light field microscope is increased by increasing the field of view and image circle diameter of the microscope, while keeping the ratio of overall magnification of the microscope to the numerical aperture of the microscope unchanged. Alternatively, the information budget is increased by increasing the field of view and image circle diameter of the microscope by a first factor, while increasing the ratio of overall magnification of the microscope to the numerical aperture of the microscope by a smaller, second factor. In some cases, an infinity-corrected light field microscope has an overall magnification that is greater than the nominal magnification of the objective lens. ## Claims ### Claim 1 A microscope comprising: (a) an infinity-corrected objective lens that has a focal length; (b) a tube lens for refracting light from the objective lens, which tube lens has a focal length that is longer than 200 millimeters; (c) a set of one or more lenslet arrays; and (d) one or more digital image sensors, which image sensors are configured for capturing light field images; wherein (i) each respective lenslet array is positioned in such a way that light from the tube lens passes through the respective lenslet array and travels to a digital image sensor, and (ii) the microscope has an overall magnification that is substantially greater than a nominal magnification of the objective lens, which nominal magnification is equal to 200 millimeters divided by the focal length of the objective lens. ### Claim 2 (depends on 1) The microscope of claim 1, wherein the tube lens has a focal length of at least 225 millimeters. ### Claim 3 (depends on 1) The microscope of claim 1, wherein the tube lens has a focal length of at least 300 millimeters. ### Claim 4 (depends on 1) The microscope of claim 1, wherein: (a) the set of lenslet arrays includes a first lenslet array and a second lenslet array; (b) the first lenslet array is at first optical distance from the objective lens and the second lenslet array is at a second optical distance from the objective lens; (c) the first optical distance is not equal to the second optical distance; (d) the first lenslet array is positioned at a first lateral distance relative to a specific image sensor that receives light from the first lenslet array, which first lateral distance is in a direction parallel to a surface of the specific image sensor; (e) the second lenslet array is positioned at a second lateral distance relative to a particular image sensor that receives light from the second lenslet array, which second lateral distance is in a direction parallel to a surface of the particular image sensor; and (f) the first lateral distance is different than the second lateral distance. ### Claim 5 (depends on 1) The microscope of claim 1, wherein: (a) the set of lenslets arrays comprises multiple lenslet arrays; (b) each respective lenslet array in the set of lenslet arrays (i) is at an optical distance from the objective lens, which optical distance is equal to that for each other lenslet array in the set, and (ii) is positioned at a lateral distance relative to a specific image sensor that receives light from the respective lenslet array, which lateral distance is in a direction parallel to a surface of the specific image sensor; and (c) the lateral distance is different for each lenslet array in the set of lenslet arrays. ### Claim 6 (depends on 1) The microscope of claim 1, wherein: (a) each respective lenslet array is shifted by a distance relative to a specific image sensor that receives light from the respective lenslet array, which distance is in a direction parallel to a surface of the specific image sensor; (b) the distance is different for each lenslet array in the set of lenslet arrays; and (c) a computer is configured to calculate a combined digital image, by computationally combining digital images that are captured by the image sensors, such that the combined image includes overlap regions, each of which overlap regions records light that passed through lenslets of more than one lenslet array. ### Claim 7 (depends on 1) The microscope of claim 1, wherein lenslets in each lenslet array, respectively, are hexagonally packed. ### Claim 8 (depends on 1) The microscope of claim 1, wherein the focal length of the tube lens is at least 400 millimeters. ### Claim 9 A microscope comprising: (a) an infinity-corrected objective lens; (b) a tube lens for refracting light from the objective lens, which tube lens has a focal length that is longer than 200 millimeters; (c) a set of one or more lenslet arrays; and (d) one or more digital image sensors, which image sensors are configured for capturing light field images; wherein each respective lenslet array is positioned in such a way that light from the tube lens passes through the respective lenslet array and travels to a digital image sensor. ### Claim 10 (depends on 9) The microscope of claim 9, wherein: (a) the set of lenslet arrays includes a first lenslet array and a second lenslet array; (b) the first lenslet array is at first optical distance from the objective lens and the second lenslet array is at a second optical distance from the objective lens; (c) the first optical distance is not equal to the second optical distance; (d) the first lenslet array is positioned at a first lateral distance relative to a specific image sensor that receives light from the first lenslet array, which first lateral distance is in a direction parallel to a surface of the specific image sensor; (e) the second lenslet array is positioned at a second lateral distance relative to a particular image sensor that receives light from the second lenslet array, which second lateral distance is in a direction parallel to a surface of the particular image sensor; and (f) the first lateral distance is different than the second lateral distance. ### Claim 11 (depends on 9) The microscope of claim 9, wherein: (a) the set of lenslets arrays comprises multiple lenslet arrays; (b) each respective lenslet array in the set of lenslet arrays (i) is at an optical distance from the objective lens, which optical distance is equal to that for each other lenslet array in the set, and (ii) is positioned at a lateral distance relative to a specific image sensor that receives light from the respective lenslet array, which lateral distance is in a direction parallel to a surface of the specific image sensor; and (c) the lateral distance is different for each lenslet array in the set of lenslet arrays. ### Claim 12 (depends on 9) The microscope of claim 9, wherein the focal length of the tube lens is at least 225 millimeters. ### Claim 13 (depends on 9) The microscope of claim 9, wherein the lenslets in each lenslet array, respectively, are hexagonally packed.