# US 10,119,980 B2 · Method for detection of basic peptide and reagent for detection of basic peptide

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,119,980 B2
- Document ID: US10119980B2
- Status: Granted
- Kind: B2
- Application number: 15/380,220
- Filed: 2016-12-15
- Published: 2017-03-30
- Granted: 2018-11-06
- Earliest claimed: 2009-12-10
- Assignees: Sysmex Corporation; Kagoshima University
- Applicants: Sysmex Corporation; Kagoshima University
- Inventors: Hiroyuki Kabata; Hideki Takahashi; Rena Tsuruoka; Ikuro Maruyama
- Web page: https://patentgrep.com/patent/US10119980B2

## CPC

- G01N 33/74: involving hormones {or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors}
- G01N 21/82: producing a precipitate or turbidity
- G01N 33/536: with immune complex formed in liquid phase
- G01N 33/6827: {Total protein determination, e.g. albumin in urine}
- G01N 33/6872: {Intracellular protein regulatory factors and their receptors, e.g. including ion channels}
- G01N 33/86: involving blood coagulating time {or factors, or their receptors}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention provides a method for detection of a basic peptide by mixing a sample suspected to contain the basic peptide and a reagent containing denatured albumin and detecting turbidness due to a complex of the basic peptide and denatured albumin.

## Claims

### Claim 1

A method for preparing a treated liquid sample which comprises ACTH, comprising:
mixing a liquid sample comprising ACTH with a reagent solution comprising a heat-denatured albumin;
complexing the heat-denatured albumin with ACTH in the liquid mixture;
obtaining a treated liquid sample comprising aggregates of heat-denatured albumin complexed with ACTH wherein the aggregates are suitable for turbidimetry detection.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the heat denatured albumin is prepared by heating an albumin at a temperature between 100 to 130 ° C.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the heat denatured albumin is prepared by heating an albumin at a temperature between 110 to 120 ° C.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the liquid sample comprises acidic peptides and neutral peptides.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the non-aggregated portion of the treated sample comprises acidic peptides and neutral peptides, and the aggregates substantially do not comprise acidic peptides and neutral peptides.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the concentration of the heat-denatured albumin in the reagent solution is 0.2 w/v % to 5.0 w/v %.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the concentration of the heat-denatured albumin in the reagent solution is 0.2 w/v % to 0.6 w/v %.

### Claim 8 (depends on 1)

The method according to claim 1, further comprising a step of concentrating basic peptides from the liquid sample before the step of mixing.