# US 10,119,971 B2 · Detection apparatus for differential-charged particle mobility analyzer

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- Number: US 10,119,971 B2
- Document ID: US10119971B2
- Status: Granted
- Kind: B2
- Application number: 15/147,701
- Filed: 2016-05-05
- Published: 2016-08-25
- Granted: 2018-11-06
- Earliest claimed: 2008-08-07
- Assignees: Quest Diagnostics Investments Incorporated
- Applicants: Quest Diagnostics Investments Incorporated
- Inventors: Michael P. Caulfield
- Web page: https://patentgrep.com/patent/US10119971B2

## CPC

- G01N 33/582: {with fluorescent label}
- A61K 9/1275: Lipoproteins or protein-free species thereof, e.g. chylomicrons; Artificial high-density lipoproteins [HDL], low-density lipoproteins [LDL] or very-low-density lipoproteins [VLDL]; Precursors thereof
- C07K 14/775: Apolipopeptides
- G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)}
- G01N 27/624: Differential mobility spectrometry [DMS]; Field asymmetric-waveform ion mobility spectrometry [FAIMS]
- G01N 31/00: Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N 33/92: involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)}
- G01N 2021/6439: {with indicators, stains, dyes, tags, labels, marks}
- G01N 2201/06113: Coherent sources; lasers
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention provides devices and methods for identification and/or quantitation of particles through detection of fluorescence labeled particles in an apparatus for differential charged particle mobility analysis and fluorescence detection.

## Claims

### Claim 1

An apparatus for differential-charged particle mobility analysis and detection of fluorescence, the apparatus comprising:
one or more pumps adapted to transport through a capillary;
an ionizer adapted to charge particles of a fluorophore labeled sample as the sample flows within a capillary;
a differential-charged particle mobility analyzer adapted to:
receive a sample of fluorophore labeled charged particles from an ionizer; and
perform a differential-charged particle mobility analysis on a sample of charged particles; and
a fluorescence detection system adapted to detect fluorescence of the charged particles.

### Claim 2 (depends on 1)

The apparatus according to claim 1, further comprising an autosampler adapted to provide a sample for differential-charged particle mobility analysis to the one or more pumps.

### Claim 3 (depends on 1)

The apparatus according to claim 1, wherein the fluorescence detection system comprises one or more excitation sources and one or more fluorescence detectors.

### Claim 4 (depends on 3)

The apparatus according to claim 3, wherein the one or more excitation sources comprise at least one laser excitation source.

### Claim 5 (depends on 4)

The apparatus according to claim 4, wherein at least one laser excitation source operates in pulse mode.

### Claim 6 (depends on 4)

The apparatus according to claim 4, wherein at least one laser excitation source operates in continuous mode.

### Claim 7 (depends on 1)

The apparatus according to claim 1, wherein the fluorescence detection system comprises a single excitation source and a single fluorescence detector positioned on either side of a flow of the sample of charged particles.

### Claim 8 (depends on 1)

The apparatus according to claim 1, wherein the fluorescence detection system comprises an excitation source and multiple fluorescence detectors.

### Claim 9 (depends on 8)

The apparatus according to claim 8, wherein the excitation source and fluorescence detectors are arranged in an annular array positioned around a flow of the sample of charged particles.