# US 10,119,969 B2 · Compositions for the detection of microbial contaminants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,119,969 B2 - Document ID: US10119969B2 - Status: Granted - Kind: B2 - Application number: 13/095,390 - Filed: 2011-04-27 - Published: 2011-08-18 - Granted: 2018-11-06 - Earliest claimed: 2003-03-17 - Assignees: Charles River Laboratories, Inc. - Applicants: Norman R. Wainwright; Foster T. Jordan - Inventors: Norman R. Wainwright; Foster T. Jordan - Web page: https://patentgrep.com/patent/US10119969B2 ## CPC - G01N 33/579: involving limulus lysate - B01L 3/5027: {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip} - B01L 2200/16: Reagents, handling or storing thereof - B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions - B01L 2300/0867: Multiple inlets and one sample wells, e.g. mixing, dilution - B01L 2400/049: vacuum - G01N 2400/24: beta-D-Glucans, i.e. having beta 1,n (n=3,4,6) linkages between saccharide units, e.g. xanthan - G01N 2400/50: Lipopolysaccharides; LPS - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay. ## Claims ### Claim 1 A manufacture comprising a solid support having dried thereon a composition comprising a mixture of a hemocyte lysate and (i) an anti-frothing agent, (ii) a resolubilizing agent, (iii) an anti-flaking agent and an anti-frothing agent, (iv) an anti-flaking agent and a resolubilizing agent, (v) an anti-frothing agent and a resolubilizing agent, or (vi) an anti-flaking agent, an anti-frothing agent and a resolubilizing agent, wherein said lysate is capable of being solubilized or reconstituted by a sample. ### Claim 2 (depends on 1) The manufacture of claim 1, wherein said composition further comprises a substrate. ### Claim 3 (depends on 1) The manufacture of claim 1, wherein said composition comprises a hemocyte lysate and an anti-frothing agent. ### Claim 4 (depends on 1) The manufacture of claim 1, wherein said composition comprises a hemocyte lysate and a resolubilizing agent. ### Claim 5 (depends on 1) The manufacture of claim 1, wherein said composition comprises a hemocyte lysate, an anti-flaking agent, and an anti-frothing agent. ### Claim 6 (depends on 1) The manufacture of claim 1, wherein said composition comprises a hemocyte lysate, an anti-flaking agent, and a resolubilizing agent. ### Claim 7 (depends on 1) The manufacture of claim 1, wherein said composition comprises a hemocyte lysate, an anti-frothing agent, and a resolubilizing agent. ### Claim 8 (depends on 1) The manufacture of claim 1, wherein said composition comprises a hemocyte lysate, an anti-flaking agent, an anti-frothing agent, and a resolubilizing agent. ### Claim 9 A manufacture comprising a solid support comprising glass or plastic having dried thereon a composition comprising mixture of a hemocyte a lysate and (i) an anti-flaking agent, (ii) an anti-frothing agent, (iii) a resolubilizing agent, (iv) an anti-flaking agent and an anti-frothing agent, (v) an anti-flaking agent and a resolubilizing agent, (vi) an anti-frothing agent and a resolubilizing agent, or (vii) an anti-flaking agent, an anti-frothing agent and a resolubilizing agent, wherein said lysate is capable of being solubilized or reconstituted by a sample. ### Claim 10 (depends on 9) The manufacture of claim 9, wherein said composition further comprises a substrate. ### Claim 11 (depends on 9) The manufacture of claim 9, wherein said composition comprises a hemocyte lysate and an anti-flaking agent. ### Claim 12 (depends on 9) The manufacture of claim 9, wherein said composition comprises a hemocyte lysate and an anti-frothing agent. ### Claim 13 (depends on 9) The manufacture of claim 9, wherein said composition comprises a hemocyte lysate and a resolubilizing agent. ### Claim 14 (depends on 9) The manufacture of claim 9, wherein said composition comprises a hemocyte lysate, an anti-flaking agent, and an anti-frothing agent. ### Claim 15 (depends on 9) The manufacture of claim 9, wherein said composition comprises a hemocyte lysate, an anti-flaking agent, and a resolubilizing agent. ### Claim 16 (depends on 9) The manufacture of claim 9, wherein said composition comprises a hemocyte lysate, an anti-frothing agent, and a resolubilizing agent. ### Claim 17 (depends on 9) The manufacture of claim 9, wherein said composition comprises a hemocyte lysate, an anti-flaking agent, an anti-frothing agent, and a resolubilizing agent. ### Claim 18 (depends on 1) The manufacture of claim 1, wherein said anti-frothing agent is polyvinyl alcohol or polypropylene glycol. ### Claim 19 (depends on 1) The manufacture of claim 1, wherein said resolubilizing agent is mannitol, mannose, sorbitol, trehalose, maltose, dextrose, sucrose, or another monosaccharide. ### Claim 20 (depends on 1) The manufacture of claim 1, wherein said resolubilizing agent is not a disaccharide. ### Claim 21 (depends on 1) The manufacture of claim 1, wherein said anti-flaking agent is selected from the group consisting of polyethylene glycol, polyvinyl pyrolidone, polyvinyl alcohol, mannitol, or a protein. ### Claim 22 (depends on 1) The manufacture of claim 1, wherein said anti-flaking agent is not dextran.