# US 10,119,962 B2 · Sample analysis device and capturing method for exosomes

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- Number: US 10,119,962 B2
- Document ID: US10119962B2
- Status: Granted
- Kind: B2
- Application number: 14/875,015
- Filed: 2015-10-05
- Published: 2016-02-04
- Granted: 2018-11-06
- Earliest claimed: 2013-04-09
- Assignees: JVC KENWOOD Corporation
- Applicants: Jvc Kenwood Corporation
- Inventors: Makoto Itonaga; Shingo Yagyu; Yuichi Hasegawa; Koji Tsujita; Masayuki Ono
- Web page: https://patentgrep.com/patent/US10119962B2

## CPC

- G01N 33/5302: {Apparatus specially adapted for immunological test procedures}
- G01N 33/487: of liquid biological material
- G01N 33/536: with immune complex formed in liquid phase
- G01N 33/54313: {the carrier being characterised by its particulate form}
- G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A recessed portion and a protruding portion arranged periodically are formed on a base portion. In the recessed portion, an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes. The width of the protruding portion is smaller than the average particle diameter of the exosomes.

## Claims

### Claim 1

A sample analysis device, comprising:
a base portion;
a recessed portion which is formed on the base portion and in which an antibody that binds to an antigen existing on a surface of each of a plurality of exosomes to be detected is immobilized and then caused to bind to the exosomes, wherein said exosomes have an average particle diameter of around 100 nm; and
a protruding portion adjacent to the recessed portion,
wherein the recessed portion and the protruding portion are periodically arranged on the base portion,
wherein the protruding portion has a width smaller than the average particle diameter of the exosomes,
and wherein the recessed portion has a width smaller than four times the average particle diameter of the exosomes.

### Claim 2 (depends on 1)

The sample analysis device according to claim 1, wherein the recessed portion has a width larger than the average particle diameter of the exosomes and is smaller than twice a diameter of beads modifying the exosomes.

### Claim 3

A sample analysis device, comprising:
a base portion;
a recessed portion which is formed on the base portion and in which an antibody that binds to an antigen existing on a surface of each of a plurality of exosomes to be detected is immobilized and then caused to bind to the exosomes, said exosomes having an average particle diameter of 100 nm; and
a protruding portion adjacent to the recessed portion,
wherein the recessed portion and the protruding portion are periodically arranged on the base portion,
wherein the protruding portion has a width smaller than a diameter of beads modifying the exosomes,
and wherein the recessed portion has a width smaller than four times the average particle diameter of the exosomes.

### Claim 4 (depends on 3)

The sample analysis device according to claim 3, wherein the recessed portion has a width larger than an average particle diameter of the exosomes and is smaller than twice the diameter of the beads.