# US 10,119,958 B2 · Arrays and methods for guided cell patterning > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,119,958 B2 - Document ID: US10119958B2 - Status: Granted - Kind: B2 - Application number: 14/835,224 - Filed: 2015-08-25 - Published: 2016-02-25 - Granted: 2018-11-06 - Earliest claimed: 2007-01-04 - Assignees: University of Washington - Applicants: University of Washington - Inventors: Miqin Zhang; Mandana Veiseh - Web page: https://patentgrep.com/patent/US10119958B2 ## CPC - G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics} - C40B 20/02: Identifying library members by their fixed physical location on a support or substrate - C40B 40/02: Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors - G01N 33/5005: {involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025)} - H10P 14/69215: {the material being a silicon oxide, e.g. SiO2} - C40B 60/04: Integrated apparatus specially adapted for both screening libraries and identifying library members - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60) - H10P: GENERIC PROCESSES OR APPARATUS FOR THE MANUFACTURE OR TREATMENT OF DEVICES COVERED BY CLASS H10 ## Abstract Guided cell patterning arrays for single cell patterning are disclosed. The arrays include a plurality of cell adhesion sites that are individually isolated on an inert surface. Each cell adhesion site has one or more cell adhesion peptides having affinity to a cell surface receptor. The inert surface is resistant to cell adhesion. ## Claims ### Claim 1 An array for guided cell patterning, comprising a plurality of individually immobilized cells isolated on an inert surface resistant to cell adhesion, wherein the inert surface comprises a silicon oxide surface having polyalkylene oxide moieties covalently coupled thereto, wherein the silicon oxide surface comprises from about 40% to 65% by weight Si, from about 5% to about 20% by weight SiOx<2, and from about 20% to about 40% by weight SiO2. ### Claim 2 (depends on 1) The array of claim 1, wherein the silicon oxide surface comprises from about 50% to 60% by weight Si, from about 10% to about 15% by weight SiOx<2, and from about 25% to about 35% by weight SiO2. ### Claim 3 (depends on 1) The array of claim 1, wherein the silicon oxide surface comprises about 58% by weight Si, about 12% by weight SiOx<2, and about 30% by weight SiO2. ### Claim 4 (depends on 1) The array of claim 1, wherein each cell is immobilized through the interaction of one or more ligands and one or more cell surface receptors of the immobilized cell. ### Claim 5 (depends on 4) The array of claim 4, wherein the one or more ligands are covalently coupled to a self-assembly monolayer on a metal surface on the inert surface. ### Claim 6 (depends on 4) The array of claim 4, wherein the one or more ligands are adsorbed to a self-assembly monolayer on a metal surface on the inert surface. ### Claim 7 (depends on 4) The array of claim 4, wherein the one or more ligands are cell adhesion peptides. ### Claim 8 (depends on 7) The array of claim 7, wherein the cell adhesion peptides are selected from the group consisting of a peptide capable of binding human umbilical cord vein endothelial cells, a peptide capable of binding DAOY cells, and a peptide capable of binding glioma cells. ### Claim 9 (depends on 7) The array of claim 7, wherein the cell adhesion peptide is Lys-Arg-Glu-Asp-Val-Tyr (SEQ ID NO.: 1). ### Claim 10 (depends on 7) The array of claim 7, wherein the cell adhesion peptide is Lys-Arg-Gly-Asp (SEQ ID NO: 2). ### Claim 11 (depends on 7) The array of claim 7, wherein the cell adhesion peptide is Arg-Glu-Asp-Val-Tyr (SEQ ID NO: 3). ### Claim 12 (depends on 7) The array of claim 7, wherein the cell adhesion peptide is Arg-Gly-Asp. ### Claim 13 (depends on 4) The array of claim 4, wherein the one or more ligands are peptides. ### Claim 14 (depends on 13) The array of claim 13, wherein the peptide is chlorotoxin. ### Claim 15 (depends on 13) The array of claim 13, wherein the peptide is nerve growth factor. ### Claim 16 (depends on 4) The array of claim 4, wherein the one or more ligands are cell adhesion proteins or fragments thereof. ### Claim 17 (depends on 16) The array of claim 16, wherein the cell adhesion protein is fibronectin or a fragment thereof. ### Claim 18 (depends on 1) The array of claim 1, wherein the plurality of individually immobilized cells are selected from the group consisting of endothelial cells, DAOY cells, glioma cells, macrophages, stem cells, bone cells, muscle cells, and nerve cells. ### Claim 19 (depends on 18) The array of claim 18, wherein the endothelial cells are human umbilical cord vein endothelial (HUVE) cells. ### Claim 20 (depends on 18) The array of claim 18, wherein the stem cells are mesenchymal stem cells. ### Claim 21 An array for guided cell patterning, comprising a plurality of individually immobilized cells isolated on an inert surface resistant to cell adhesion, wherein the inert surface comprises a silicon oxide surface having polyalkylene oxide moieties covalently coupled thereto, wherein each cell is immobilized through the interaction of one or more peptide ligands and one or more cell surface receptors of the immobilized cell, wherein the one or more peptide ligands is chlorotoxin. ### Claim 22 An array for guided cell patterning, comprising a plurality of individually immobilized cells isolated on an inert surface resistant to cell adhesion, wherein the inert surface comprises a silicon oxide surface having polyalkylene oxide moieties covalently coupled thereto, wherein each cell is immobilized through the interaction of one or more peptide ligands and one or more cell surface receptors of the immobilized cell, wherein the one or more peptide ligands is nerve growth factor.