# US 10,119,947 B2 · Protein-rich microalgal biomass compositions of optimized sensory quality > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,119,947 B2 - Document ID: US10119947B2 - Status: Granted - Kind: B2 - Application number: 14/910,918 - Filed: 2014-08-06 - Published: 2016-07-07 - Granted: 2018-11-06 - Earliest claimed: 2013-08-07 - Assignees: Corbion Biotech, Inc. - Applicants: Corbion Biotech, Inc. - Inventors: Heike Jerosch; Amandine Druon; Marilyne Guillemant; Samuel Patinier - Web page: https://patentgrep.com/patent/US10119947B2 ## CPC - G01N 33/0001: {by organoleptic means} - A23J 1/009: {from unicellular algae (seaweed A23J1/006)} - G01N 30/7206: {interfaced to gas chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04)} - A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs - C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00) - G01N 33/02: Food - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS - A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract The invention relates to a method for determining the organoleptic quality of a protein-rich microalgal biomass composition, comprising the determination of the content of 11 volatile organic compounds, wherein the 11 volatile organic compounds are pentanal, hexanal, 1-octen-3-ol, 2-pentylfuran, octanal, 3,5-octadien-2-ol (or 3-octen-2-one), 3,5-octadien-2-one, nonanal, 2-no-nenal, (E,E)-2,4-nonadienal and hexanoic acid. ## Claims ### Claim 1 A method for determining the organoleptic quality of a protein-rich microalgal biomass composition, comprising determining the content of 11 volatile organic compounds, the 11 volatile organic compounds being pentanal, hexanal, 1-octen-3-ol, 2-pentylfuran, octanal, 3,5-octadien-2-ol (or 3-octen-2-one), 3,5-octadien-2-one, nonanal, 2-nonenal, (E,E)-2,4-nonadienal and hexanoic acid, characterized in that the microalgal biomass comprises more than 50% proteins by dry weight of biomass and in that the microalgae are of the Chlorella genus. ### Claim 2 (depends on 1) The method as claimed in claim 1, wherein the content of 11 volatile organic compounds is determined by SPME/GC of by SPME/GC-MS. ### Claim 3 (depends on 2) The method as claimed in claim 2, characterized in that the content of 11 volatile organic compounds is determined by the surface area of the chromatography peaks after SPME/GC. ### Claim 4 (depends on 1) The method as claimed in claim 1, characterized in that the content of 11 volatile organic compounds is compared to that of a reference protein-rich microalgal biomass or biomasses for which the organoleptic qualities are defined. ### Claim 5 (depends on 4) The method as claimed in claim 4, wherein the content of the 11 volatile organic compounds is determined by the surface area of the chromatography peaks corresponding to the 11 volatile organic compounds and compared to the content of the 11 volatile organic compounds in the reference protein-rich microalgal biomass or biomasses for which the organoleptic qualities are defined. ### Claim 6 (depends on 1) The method as claimed in claim 1, characterized in that the microalgae are selected from the group consisting of Chlorella vulgaris, Chlorella sorokiniana and Chlorella protothecoides. ### Claim 7 (depends on 6) The method as claimed in claim 6, wherein the microalgae is Chlorella protothecoides. ### Claim 8 A method for defining an analytical profile of volatile organic compounds making it possible to evaluate the organoleptic quality of the protein-rich microalgal biomass compositions, comprising: the construction of a first matrix associating microalgal biomass compositions, including two controls of acceptable and unacceptable organoleptic quality, with the evaluation of their organoleptic qualities by a sensory panel of at least 15 individuals, the construction of a second matrix associating with these same compositions their characterization by a volatile organic compound analysis profile, and the correlation of the first matrix with the second to produce a relationship model on the basis of which the compositions having an optimized organoleptic profile can thus be characterized by their analytical profile of volatile organic compounds; characterized in that the microalgal biomass comprises more than 50% proteins by dry weight of biomass and in that the microalgae are of the Chlorella genus. ### Claim 9 (depends on 8) The method as claimed in claim 8, characterized in that the descriptors of the sensory analysis comprise: the following odors: vegetable, mash, stock, rancid butter, cheese, manure, fermented, peanut and paint; and the following colors: yellow and green. ### Claim 10 A method for determining the organoleptic quality of a protein-rich microalgal biomass composition, comprising determining the content of 4 volatile organic compounds, these 4 organic compounds being 3,5-octadien-2-ol (or 3-octen-2-one), 1-octen-3-ol, 3,5-octadien-2-one and (E,E)-2,4-nonadienal and calculating an overall flavor value from the sum of the individual flavor values of 3,5-octadien-2-ol (or 3-octen-2-one), 1-octen-3-ol, 3,5-octadien-2-one and (E,E)-2,4-nonadienal, characterized in that the microalgal biomass comprises more than 50% proteins by dry weight of biomass and in that the microalgae are of the Chlorella genus. ### Claim 11 (depends on 10) The method as claimed in claim 10, characterized in that the microalgae are selected from the group consisting of Chlorella vulgaris, Chlorella sorokiniana and Chlorella protothecoides. ### Claim 12 (depends on 11) The method as claimed in claim 11, wherein the microalgae is Chlorella protothecoides. ### Claim 13 A method for selecting protein-rich microalgal biomass compositions having an acceptable organoleptic profile, characterized in that the organoleptic quality is determined by the method as claimed in 9, and that the composition is selected when the overall flavor value calculated by the method is between 0 and 40% relative to that of an organoleptically unacceptable reference microalgal biomass composition, characterized in that the microalgal biomass comprises more than 50% proteins by dry weight of biomass and in that the microalgae are of the Chlorella genus.