# US 10,119,914 B2 · Fast high-resolution microscopy method and fast high-resolution microscope > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,119,914 B2 - Document ID: US10119914B2 - Status: Granted - Kind: B2 - Application number: 15/380,880 - Filed: 2016-12-15 - Published: 2017-06-22 - Granted: 2018-11-06 - Earliest claimed: 2015-12-16 - Assignees: Carl Zeiss Microscopy GmbH - Applicants: Carl Zeiss Microscopy GmbH - Inventors: Thomas Kalkbrenner - Web page: https://patentgrep.com/patent/US10119914B2 ## CPC - G01N 21/6458: {Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16)} - G02B 21/0004: {specially adapted for specific applications} - G02B 21/16: adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)} - G02B 21/367: {providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS ## Abstract A fast, high-resolution localization microscopy method for a specimen containing fluorescence emitters. In a bright state, emitters emit fluorescent radiation upon excitation irradiation, and in a dark state, the fluorescence emitters do not emit fluorescent radiation upon excitation irradiation. The emitters can be brought from the bright state into the dark state by irradiation, and the dark state has a lifetime after which the emitters return to the bright state spontaneously, such that the emitters blink at a blinking frequency. The blinking specimen is imaged with a spatial resolution and detected with a camera, the intensity of the radiation is set such that emitters are isolated in the individual images in terms of the spatial resolution, and the refresh rate at which the images are produced is not lower than the blinking frequency. Locations are determined for isolated emitters in the images with an accuracy that exceeds the spatial resolution. ## Claims ### Claim 1 A fast, high-resolution microscopy method of a specimen containing a fluorescence emitter, comprising: using in a specimen, fluorescence emitters that have a first and a second state, wherein the first state is a bright state in which the fluorescence emitters emit fluorescent radiation on irradiation of excitation radiation, and the second state is a dark state in which the fluorescence emitters do not emit fluorescent radiation on irradiation of excitation radiation, wherein the fluorescence emitters can be brought from the bright state into the dark state by irradiation of excitation radiation and the dark state has a particular lifetime after which the fluorescence emitters return to the bright state spontaneously, illuminating the specimen and the fluorescence emitters in the widefield with excitation radiation having an intensity, thereby exciting the fluorescence emitters and causing the fluorescence emitters to blink, such that the fluorescence emitters switch back and forth between a bright state and a dark state at a blinking frequency, detecting and imaging with a camera the blinking specimen in the widefield with a spatial resolution, with a camera, setting the intensity of the excitation radiation such that at least some fluorescence emitters are isolated in the individual images in terms of the spatial resolution, and the intensity of the excitation radiation and a refresh rate of the camera are adapted to each other such that the refresh rate with which the individual images are produced is not lower than the blinking frequency, and subjecting the individual images to a localization analysis, wherein locations are determined for isolated fluorescence emitters in the individual images with an accuracy that exceeds the spatial resolution. ### Claim 2 (depends on 1) The microscopy method according to claim 1, wherein an integration time for the production of an individual image is not longer than the lifetime of the dark state. ### Claim 3 (depends on 1) The microscopy method according to claim 1, wherein, in a test measurement, the excitation radiation is irradiated in a pulsed manner, the lifetime of the dark state is measured and the refresh rate is set correspondingly, with the result that an integration time is 10% of the lifetime of the dark state. ### Claim 4 (depends on 1) The microscopy method according to claim 1, wherein the integration time is not more than 300 μs. ### Claim 5 (depends on 1) The microscopy method according to claim 1, wherein the fluorescence emitters are in a chemically unaffected state with respect to the lifetime of the dark state. ### Claim 6 (depends on 1) The microscopy method according to claim 1, wherein an intensity of the excitation radiation does not exceed a value of 1 MW/cm2. ### Claim 7 (depends on 1) The microscopy method according to claim 1, wherein the intensity of the excitation radiation is set such that the blinking frequency has a value of not less than 1/(300 μs). ### Claim 8 (depends on 1) The microscopy method according to claim 1, wherein in the production of the individual images the excitation radiation is irradiated as CW radiation. ### Claim 9 A high-resolution microscope for fast microscopy of a specimen containing a fluorescence emitter, comprising: an excitation device that is designed to excite the specimen in the widefield with excitation radiation to emit fluorescent radiation, an imaging device that is designed to image the specimen in the widefield with a spatial resolution, wherein the excitation device is designed such that at least some fluorescence emitters are isolated in the imaging in terms so the spatial resolution, a localization analysis device that is designed to determine locations for the isolated fluorescence emitters with an accuracy that exceeds the spatial resolution, and a control device that is designed to control the excitation apparatus and the imaging apparatus in accordance with the following method: fast high-resolution microscopy method of a specimen containing a fluorescence emitter, in which fluorescence emitters are used that have a first and a second state, wherein the first state is a bright state in which the fluorescence emitters emit fluorescent radiation on irradiation of excitation radiation, and the second state is a dark state in which the fluorescence emitters do not emit fluorescent radiation on irradiation of excitation radiation, wherein the fluorescence emitters can be brought from the bright state into the dark state by irradiation of excitation radiation and the dark state has a particular lifetime after which the fluorescence emitters return to the bright state spontaneously, the specimen and thus the fluorescence emitters are illuminated in the widefield with excitation radiation of an intensity and the fluorescence emitters are thereby excited to blink, such that they switch back and forth between bright state and dark state at a blinking frequency, the blinking specimen is imaged in the widefield with a spatial resolution and is detected with a camera, the intensity of the excitation radiation is set such that at least some fluorescence emitters are isolated in the individual images in terms of the spatial resolution, and the intensity of the excitation radiation and a refresh rate of the camera are adapted to each other such that the refresh rate with which the individual images are produced is not lower than the blinking frequency, and the individual images are subjected to a localization analysis, wherein locations are determined for isolated fluorescence emitters in the individual images with an accuracy that exceeds the spatial resolution. ### Claim 10 (depends on 9) The microscope according to claim 9, wherein the imaging device comprises a CMOS two-dimensional detector with an image intensifier connected upstream in the beam path. ### Claim 11 (depends on 9) The microscope according to claim 9, wherein the refresh rate has a value of not less than 1/(300 μs). ### Claim 12 (depends on 9) The microscope according to claim 9, wherein the imaging device comprises a SPAD array as two-dimensional detector. ### Claim 13 (depends on 11) The microscope according to claim 11, which comprises an image field displacement device in order to image a specimen field by displacing an image field onto the SPAD array and thus to assemble each individual image from several partial individual images. ### Claim 14 (depends on 1) The microscopy method according to claim 1, wherein, in a test measurement, the excitation radiation is irradiated in a pulsed manner, the lifetime of the dark state is measured and the refresh rate is set correspondingly, with the result that an integration time is 50% of the lifetime of the dark state. ### Claim 15 (depends on 1) The microscopy method according to claim 1, wherein, in a test measurement, the excitation radiation is irradiated in a pulsed manner, the lifetime of the dark state is measured and the refresh rate is set correspondingly, with the result that an integration time is 90% of the lifetime of the dark state. ### Claim 16 (depends on 1) The microscopy method according to claim 1, wherein the integration time is not more than 100/μs. ### Claim 17 (depends on 1) The microscopy method according to claim 1, wherein the integration time is not more than 50/μs. ### Claim 18 (depends on 1) The microscopy method according to claim 1, wherein the integration time is not time between 1 μs more than 30 μs. ### Claim 19 (depends on 1) The microscopy method according to claim 1, wherein an intensity of the excitation radiation does not exceed a value of 50 MW/cm2. ### Claim 20 (depends on 1) The microscopy method according to claim 1, wherein an intensity of the excitation radiation does not exceed a value of 2 MW/cm2. ### Claim 21 (depends on 1) The microscopy method according to claim 1, wherein the intensity of the excitation radiation is set such that the blinking frequency has a value of not less than 1/(50 μs). ### Claim 22 (depends on 1) The microscopy method according to claim 1, wherein the intensity of the excitation radiation is set such that the blinking frequency has a value of not less than 1/(1 μs). ### Claim 23 (depends on 9) The microscope according to claim 9, wherein the refresh rate has a value of not less than 1/(100 μs). ### Claim 24 (depends on 9) The microscope according to claim 9, wherein the refresh rate has a value of not less than 1/(150 μs). ### Claim 25 (depends on 9) The microscope according to claim 9, wherein the refresh rate has a value between 1/(30 μs) and 1 μs.