# US 10,119,898 B2 · Particle screening device

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- Number: US 10,119,898 B2
- Document ID: US10119898B2
- Status: Granted
- Kind: B2
- Application number: 15/326,425
- Filed: 2015-06-24
- Published: 2017-07-20
- Granted: 2018-11-06
- Earliest claimed: 2014-07-22
- Assignees: Pixel Biotech (Shanghai) Co., Ltd.
- Applicants: Pixel Biotech (Shanghai) Co., Ltd.
- Inventors: Chengjun Huang; Jun Luo; Chao Zhao
- Web page: https://patentgrep.com/patent/US10119898B2

## CPC

- G01N 15/1023: {Microstructural devices for non-optical measurement}
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 3/50273: {characterised by the means or forces applied to move the fluids}
- B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules}
- C12M 23/16: {Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093)}
- C12M 35/02: {Electrical or electromagnetic means, e.g. for electroporation or for cell fusion}
- C12M 47/04: {Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04)}
- G01N 15/1031: by measuring electrical or magnetic effects
- G01N 33/575: for cancer
- B01L 2200/0652: Sorting or classification of particles or molecules
- B01L 2300/0645: Electrodes
- B01L 2300/0887: Laminated structure
- B01L 2400/0424: Dielectrophoretic forces
- G01N 15/01: specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05)
- G01N 2015/1006: {for cytology}
- G01N 2015/1028: {Sorting particles}
- G01N 2015/1029: {Particle size}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

A particle screening device is provided. The particle screening device comprises: a substrate including a first side and a second side opposite to the first side; a micropore array formed on the substrate, wherein each micropore penetrates through the substrate from the first side to the second side and has a size configured to at least permit particles smaller than target particles flow through; and electrodes formed on at least one side of the first and second sides of the substrate and around at least some micropores, wherein the electrodes are configured to generate an electric field at corresponding micropores.

## Claims

### Claim 1

A particle screening device, comprising:
a substrate including a first side and a second side opposite to the first side;
a micropore array formed on the substrate, wherein each micropore penetrates through the substrate from the first side to the second side and has a size configured to at least permit particles smaller than target particles to flow into the micropore at the first side of the substrate, through the micropore, and flow out of the micropore at the second side of the substrate; and
electrodes formed on at least one side of the first and second sides of the substrate and around at least some micropores, wherein the electrodes are configured to generate an electric field at corresponding micropores so as to achieve DEP of the target particles in the electric field, such that the target particles are attracted to and captured at the micropores while the particles smaller than the target particles flow out of the micropore at the second side of the substrate.

### Claim 2 (depends on 1)

The particle screening device of claim 1, wherein the substrate comprises at least one of silicon, glass, quartz and polymer.

### Claim 3 (depends on 1)

The particle screening device of claim 1, wherein the micropores have a size configured to be larger than at least some target particles.

### Claim 4 (depends on 1)

The particle screening device of claim 1, wherein the target particles comprise CTCs.

### Claim 5 (depends on 4)

The particle screening device of claim 4, wherein the micropores have a size of about 100 nm-100 μm.

### Claim 6 (depends on 1)

The particle screening device of claim 1, wherein the electric field is determined by at least one of the size and the dielectric property of the target particles so as to achieve DEP of the target particles in the electric field.

### Claim 7 (depends on 1)

The particle screening device of claim 1, wherein the electrodes are configured to be applied a direct voltage or an alternating voltage.

### Claim 8 (depends on 7)

The particle screening device of claim 7, wherein the applied voltage has an amplitude of 0V-100V, and the applied alternating voltage has a frequency of 0 Hz-200 MHz.

### Claim 9 (depends on 1)

The particle screening device of claim 1, wherein the electrodes are configured to be applied a voltage to lyse the target particles.

### Claim 10 (depends on 1)

The particle screening device of claim 1, wherein the electrodes comprise a first electrode and a second electrode disposed and separated along a periphery of a corresponding micropore, and wherein the first and second electrodes are configured to generate an electric field therebetween.

### Claim 11 (depends on 10)

The particle screening device of claim 10, further comprising wirings for applying voltages to the first and second electrodes respectively.

### Claim 12 (depends on 11)

The particle screening device of claim 11, wherein the micropores in the array are arranged by lines and columns, and wherein the first electrodes of the micropores in the same line are connected to one wiring, and the second electrodes of the micropores in the same column are connected to one further wiring.

### Claim 13 (depends on 10)

The particle screening device of claim 10, further comprising:
bonding pads for applying voltages to the first and second electrodes at respective micropores; and
wirings formed between respective bonding pads and corresponding electrodes.

### Claim 14 (depends on 1)

The particle screening device of claim 1, wherein the electrodes have a stack structure of a first electrode layer, a dielectric layer and a second electrode layer, and wherein the stack structure is configured to generate an electric field between the first electrode layer and the second electrode layer.

### Claim 15 (depends on 1)

The particle screening device of claim 1, wherein the electrodes are formed with respect to respective micropores, and the electrode of each micropore can be addressed individually.

### Claim 16 (depends on 1)

The particle screening device of claim 1, further comprising a micro-fluidic chip which is configured to introduce fluidic samples containing the target particles.