# US 10,119,892 B2 · Automatic tissue staining device and automatic tissue staining method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,119,892 B2 - Document ID: US10119892B2 - Status: Granted - Kind: B2 - Application number: 15/511,481 - Filed: 2015-09-18 - Published: 2017-10-12 - Granted: 2018-11-06 - Earliest claimed: 2014-09-22 - Assignees: Nichirei Biosciences Inc. - Applicants: Nichirei Biosciences Inc. - Inventors: Toshiyuki Kasamatsu; Noboru Horikoshi - Web page: https://patentgrep.com/patent/US10119892B2 ## CPC - G01N 1/312: {for samples mounted on planar substrates} - G01N 1/28: Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) - G01N 1/30: Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} - G01N 1/31: Apparatus therefor - G01N 33/48: Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) - G01N 35/00029: {provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence)} - G01N 35/00722: {Communications; Identification} - G01N 35/0095: {introducing urgent samples with priority, e.g. Short Turn Around Time Samples [STATS]} - G01N 2035/00138: {Slides} - G01N 2035/00356: {Holding samples at elevated temperature (incubation)} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract An automatic tissue staining device includes a supply head that supplies a treatment fluid, a horizontal direction movement unit that moves the supply head in a horizontal direction, and a holding unit that holds a plurality of glass slides on which samples are set. The automatic tissue staining device further includes a control unit that judges an occupancy status of the horizontal direction movement unit in a condition that one or more of the glass slides are situated in a first region prior to supplying the treatment fluid from the supply head to one or more samples in the first region, suspends a start of a treatment for the samples in the first region when the horizontal direction movement unit is occupied, and permits the treatment for the samples in the first region when the horizontal direction movement unit is not occupied. ## Claims ### Claim 1 An automatic tissue staining device comprising: a supply head configured to supply a treatment fluid; a horizontal direction movement unit configured to move the supply head in a horizontal direction; a holding unit configured to hold a plurality of glass slides on which samples are set, the holding unit including a first region and a second region different from the first region; and a control unit configured to judge an occupancy status of the horizontal direction movement unit in a condition that one or more of the glass slides are situated in the first region, prior to supply of the treatment fluid from the supply head to one or more samples on the one or more glass slides situated in the first region; suspend a start of a soonest treatment for the one or more samples on the one or more glass slides situated in the first region when the horizontal direction movement unit is occupied; and permit the start of the soonest treatment for the one or more samples on the one or more glass slides situated in the first region when the horizontal direction movement unit is not occupied. ### Claim 2 (depends on 1) The automatic tissue staining device according to claim 1, further comprising: a heating unit for heating the samples on the plurality of glass slides; a temperature measurement unit for measuring a temperature of the samples heated by the heating unit; and an accumulation unit configured to judge that the temperature of the samples has reached a prescribed temperature according to a result of measurement by the temperature measurement unit, start a count, and perform accumulation, wherein: the treatment fluid includes a treatment liquid, and the accumulation unit is configured to start the count when the samples on the plurality of glass slides are supplemented with the treatment liquid and restart the count when the prescribed temperature is reached after supplementation with the treatment liquid. ### Claim 3 (depends on 2) The automatic tissue staining device according to claim 2, wherein: one or more of the glass slides are situated in the second region, the control unit is configured to make a comparison between a usage schedule of the horizontal direction movement unit in a case where a treatment for one or more first samples on the one or more glass slides situated in the first region is started and a usage schedule of the horizontal direction movement unit in a treatment for one or more second samples on the one or more glass slides situated in the second region, and thereby judge whether or not there is an overlap between a time of using the horizontal direction movement unit in the case where the treatment for the one or more first samples is started and a time of using the horizontal direction movement unit in the treatment for the one or more second samples, when there is an overlap between the time of using the horizontal direction movement unit in the case where the treatment for the one or more first samples is started and the time of using the horizontal direction movement unit in the treatment for the one or more second samples, the control unit is configured to make a comparison between contents of a treatment schedule for the one or more first samples and contents of a treatment schedule for the one or more second samples in the overlapping time, when the contents of the treatment schedule for the one or more first samples have priority over the contents of the treatment schedule for the one or more second samples, the control unit is configured to permit the start of the soonest treatment for the one or more first samples using the horizontal direction movement unit, and when the contents of the treatment schedule for the one or more second samples have priority over the contents of the treatment schedule for the one or more first samples, the control unit is configured to suspend the start of the soonest treatment for the one or more first samples using the horizontal direction movement unit. ### Claim 4 (depends on 3) The automatic tissue staining device according to claim 3, wherein when the contents of the treatment schedule for the one or more first samples are a treatment of stopping a reaction upon the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment other than a treatment of stopping a reaction upon the one or more second samples, the contents of the treatment schedule for the one or more first samples are prioritized over the contents of the treatment schedule for the one or more second samples. ### Claim 5 (depends on 4) The automatic tissue staining device according to claim 4, wherein when the contents of the treatment schedule for the one or more first samples are a treatment other than a treatment of stopping a reaction upon the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment other than a treatment of stopping a reaction upon the one or more second samples and a time for which the soonest treatment to be performed for the one or more first samples by using the horizontal direction movement unit has been suspended is longer than a time for which the soonest treatment to be performed for the one or more second samples by using the horizontal direction movement unit has been suspended, the contents of the treatment schedule for the one or more first samples are prioritized over the contents of the treatment schedule for the one or more second samples. ### Claim 6 (depends on 5) The automatic tissue staining device according to claim 5, wherein when the contents of the treatment schedule for the one or more first samples are a treatment of stopping the reaction upon the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment of stopping the reaction upon the one or more second samples, the contents of the treatment schedule for the one or more first samples are prioritized over the contents of the treatment schedule for the one or more second samples if a reaction time specified for the one or more first samples being stoppage targets is shorter than a reaction time specified for the one or more second samples being stoppage targets. ### Claim 7 (depends on 6) The automatic tissue staining device according to claim 6, wherein when the one or more first samples and the one or more second samples are samples for which an immunostaining method has been specified as a staining method and the contents of the treatment schedule for the one or more first samples are a treatment of stopping the reaction upon the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment of stopping the reaction upon the one or more second samples, the contents of the treatment schedule for the one or more first samples are prioritized over the contents of the treatment schedule for the one or more second samples if the contents of the treatment schedule for the one or more first samples are a treatment of stopping a reaction of a primary antibody upon an antigen in the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment other than a treatment of stopping a reaction of a primary antibody upon an antigen in the one or more second samples. ### Claim 8 (depends on 3) The automatic tissue staining device according to claim 3, wherein when the contents of the treatment schedule for the one or more first samples are a treatment other than a treatment of stopping a reaction upon the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment other than a treatment of stopping a reaction upon the one or more second samples and a time for which the soonest treatment to be performed for the one or more first samples by using the horizontal direction movement unit has been suspended is longer than a time for which the soonest treatment to be performed for the one or more second samples by using the horizontal direction movement unit has been suspended, the contents of the treatment schedule for the one or more first samples are prioritized over the contents of the treatment schedule for the one or more second samples. ### Claim 9 (depends on 8) The automatic tissue staining device according to claim 8, wherein when the contents of the treatment schedule for the one or more first samples are a treatment of stopping the reaction upon the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment of stopping the reaction upon the one or more second samples, the contents of the treatment schedule for the one or more first samples are prioritized over the contents of the treatment schedule for the one or more second samples if a reaction time specified for the one or more first samples being stoppage targets is shorter than a reaction time specified for the one or more second samples being stoppage targets. ### Claim 10 (depends on 8) The automatic tissue staining device according to claim 8, wherein when the one or more first samples and the one or more second samples are samples for which an immunostaining method has been specified as a staining method and the contents of the treatment schedule for the one or more first samples are a treatment of stopping the reaction upon the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment of stopping the reaction upon the one or more second samples, the contents of the treatment schedule for the one or more first samples are prioritized over the contents of the treatment schedule for the one or more second samples if the contents of the treatment schedule for the one or more first samples are a treatment of stopping a reaction of a primary antibody upon an antigen in the one or more first samples and the contents of the treatment schedule for the one or more second samples are a treatment other than a treatment of stopping a reaction of a primary antibody upon an antigen in the one or more second samples. ### Claim 11 (depends on 3) The automatic tissue staining device according to claim 3, wherein when the treatment for the one or more second samples cannot be started and there is no overlap between a time of using the horizontal direction movement unit in the treatment schedule for the one or more first samples and a time of using the horizontal direction movement unit in the treatment schedule for the one or more second samples, the control unit is configured to permit the treatment for the one or more first samples. ### Claim 12 (depends on 3) The automatic tissue staining device according to claim 3, further comprising a plurality of lanes, wherein: the same number of holding units as lanes is provided, the lanes are provided respectively with the holding units, a certain one of the lanes corresponds to the first region, and another lane different from the certain one of the lanes corresponding to the first region corresponds to the second region. ### Claim 13 (depends on 3) The automatic tissue staining device according to claim 3, wherein the control unit is configured to compare treatment schedules from a present time in integrated process sequences generated for a plurality of regions, respectively, to each include a sequence of treatment processes for a plurality of samples in each of the plurality of regions with each other, thereby make a comparison between a usage schedule of the horizontal direction movement unit in a case where a treatment for a plurality of first samples situated in a certain region in the plurality of regions is started and a usage schedule of the horizontal direction movement unit in a treatment for a plurality of second samples situated in another region, and thereby judge whether or not there is an overlap between the time of using the horizontal direction movement unit in the case where the treatment for the plurality of first samples is started and the time of using the horizontal direction movement unit in the treatment for the plurality of second samples. ### Claim 14 (depends on 13) The automatic tissue staining device according to claim 13, wherein the integrated process sequences are generated by comparing individual process sequences, each including a plurality of treatment processes performed for the plurality of samples, respectively, with each other in each of the plurality of regions so as to consecutively perform treatment processes of the same contents on a plurality of samples on each of the plurality of regions. ### Claim 15 (depends on 14) The automatic tissue staining device according to claim 14, wherein the integrated process sequences are generated by comparing contents of treatment liquids included in the treatment fluids supplied to the respective samples in the consecutively performed treatment processes of the same contents so that samples using the same treatment liquid are consecutively supplied with the same treatment liquid. ### Claim 16 (depends on 15) The automatic tissue staining device according to claim 15, wherein the integrated process sequences are generated so as to supply the treatment liquid included in the treatment fluid to a sample whose reaction time is longer prior to a sample whose reaction time is shorter in the consecutively performed treatment processes of the same contents. ### Claim 17 (depends on 14) The automatic tissue staining device according to claim 14, wherein the integrated process sequences are generated so as to supply the treatment liquid included in the treatment fluid to a sample whose reaction time is longer prior to a sample whose reaction time is shorter in the consecutively performed treatment processes of the same contents. ### Claim 18 (depends on 14) The automatic tissue staining device according to claim 14, further comprising: a heating unit for heating the samples on the plurality of glass slides; and a temperature measurement unit for measuring a temperature of the samples heated by the heating unit, wherein the integrated process sequences are generated so as to supply a treatment liquid included in the treatment fluid to a sample whose reaction temperature is higher prior to a sample whose reaction temperature is lower in the consecutively performed treatment processes of the same contents. ### Claim 19 (depends on 3) The automatic tissue staining device according to claim 3, wherein: the control unit is configured to judge whether or not a soonest treatment for one or more second samples on the one or more glass slides situated in the second region can be started, when the soonest treatment for the one or more second samples can be started, the control unit is configured to make a comparison between contents of a soonest treatment schedule for one or more first samples on the one or more glass slides situated in the first region and contents of a soonest treatment schedule for the one or more second samples, when the contents of the soonest treatment schedule for the one or more first samples have priority over the contents of the soonest treatment schedule for the one or more second samples, the control unit is configured to permit the start of the soonest treatment for the one or more first samples using the horizontal direction movement unit, and when the contents of the soonest treatment schedule for the one or more second samples have priority over the contents of the soonest treatment schedule for the one or more first samples, the control unit is configured to suspend the start of the soonest treatment for the one or more first samples using the horizontal direction movement unit. ### Claim 20 (depends on 2) The automatic tissue staining device according to claim 2, wherein: one or more of the glass slides are situated in the second region, the control unit is configured to judge whether or not a soonest treatment for one or more second samples on the one or more glass slides situated in the second region can be started, when the soonest treatment for the one or more second samples can be started, the control unit is configured to make a comparison between contents of a soonest treatment schedule for one or more first samples on the one or more glass slides situated in the first region and contents of a soonest treatment schedule for the one or more second samples, when the contents of the soonest treatment schedule for the one or more first samples have priority over the contents of the soonest treatment schedule for the one or more second samples, the control unit is configured to permit the start of the soonest treatment for the one or more first samples using the horizontal direction movement unit, and when the contents of the soonest treatment schedule for the one or more second samples have priority over the contents of the soonest treatment schedule for the one or more first samples, the control unit is configured to suspend the start of the soonest treatment for the one or more first samples using the horizontal direction movement unit.