# US 10,119,156 B2 · Glucose production method and glucose produced by said method

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- Number: US 10,119,156 B2
- Document ID: US10119156B2
- Status: Granted
- Kind: B2
- Application number: 14/891,952
- Filed: 2014-05-19
- Published: 2016-03-31
- Granted: 2018-11-06
- Earliest claimed: 2013-05-17
- Assignees: Acteiive Corporation; Masahiko Abe; Kengo Sakaguchui; Shigeru Kido
- Applicants: Acteiive Corporation; Masahiko Abe; Kengo Sakaguchi; Shigeru Kido
- Inventors: Yoshihiro Kimura; Misato Imai; Hisayuki Kojima; Yoshihiro Kanai; Yasutaka Seki; Kenichi Aburai; Yukiko Kikuchi
- Web page: https://patentgrep.com/patent/US10119156B2

## CPC

- C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60)
- C07H 1/08: from natural products
- C07H 3/02: Monosaccharides
- C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase}
- C13K 1/02: obtained by saccharification of cellulosic materials (manufacture of fodder A23K10/32)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- C13K: SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES (chemically synthesised sugars or sugar derivatives C07H; polysaccharides, e.g. starch, derivatives thereof C08B; malt C12C; fermentation or enzyme-using processes for preparing compounds containing saccharide radicals C12P19/00; production of sucrose C13B)

## Abstract

A glucose production method is characterized in that a cellulose raw material is decomposed using a mixture of a cellulolytic enzyme, and saliva or an activating auxiliary agent derived from biological saliva. The method achieves excellent glucose yield.

## Claims

### Claim 1

A method for producing glucose, comprising:
providing a cellulose raw material;
pulverizing the cellulose raw material to obtain a fine cellulose material;
enzymolytically decomposing the fine cellulose material using a mixture of a cellulolytic enzyme and either saliva obtained from a ruminant animal or an activating auxiliary agent isolated from saliva obtained from a ruminant animal, to obtain crude glucose material; and
purifying the crude glucose material to obtain a purified glucose material.