# US 10,119,152 B2 · Fermentation process for producing chemicals > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,119,152 B2 - Document ID: US10119152B2 - Status: Granted - Kind: B2 - Application number: 15/090,293 - Filed: 2016-04-04 - Published: 2016-09-29 - Granted: 2018-11-06 - Earliest claimed: 2011-11-03 - Assignees: Vogelbusch GmbH - Applicants: Vogelbusch GmbH - Inventors: Michael Sauer; Hans Marx; Stefan Pfluegl; Diethard Mattanovich - Web page: https://patentgrep.com/patent/US10119152B2 ## CPC - C12P 7/18: polyhydric - C12N 1/205: Bacterial isolates - C12N 1/32: Processes using, or culture media containing, lower alkanols, i.e. C1 to C6 - C12N 9/0006: {acting on CH-OH groups as donors (1.1)} - C12P 7/56: Lactic acid - C12R 2001/225: Lactobacillus - Y02P 20/52: using catalysts, e.g. selective catalysts - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS - Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS ## Abstract The invention refers to a method of biotransforming a carbohydrate of a raw material into a chemical, by cultivating Lactobacillus diolivorans in the presence of the raw material to produce a chemical substance, and isolating the chemical substance in the purified form, and the use of L. diolivorans in one of a series of biotransformation methods, wherein carbohydrates from at least two different carbohydrate sources of low purity are transformed into chemicals. ## Claims ### Claim 1 A method of producing a chemical substance by biotransforming a carbohydrate into the chemical substance, comprising the steps of: culturing Lactobacillus diolivorans (L. diolivorans) in the presence of the carbohydrate to produce the chemical substance, wherein the carbohydrate is contained in a mixture of organic carbon sources and has a purity of less than Technical Grade and an ash content of at least 0.1% (w/w) in said mixture of organic carbon sources; and isolating and purifying the chemical substance. ### Claim 2 (depends on 1) The method of claim 1, wherein the mixture of organic carbon sources is selected from the group consisting of industrial glycerol, sugar cane, sugar beet, starchy plant, cellulose, hemicellulose, lignocellulose and chitin. ### Claim 3 (depends on 1) The method of claim 1, wherein the chemical substance is selected from the group consisting of 1,2-propanediol, 2-amino-1,3-propanediol, 3-hydroxybutyrate, poly-3-hydroxybutyrate, ethanol, butanol, lactic acid, citric acid, propionic acid, 3-hydroxy propionaldehyde, 3-hydroxy propionic acid, butyric acid, valeric acid, hexanoic acid, adipic acid, succinic acid, fumaric acid, malic acid, 2,5-furan dicarboxylic acid, aspartic acid, glucaric acid, gluconic acid, glutamic acid, itaconic acid, levulinic acid, acrylic acid, propanol, isopropanol, butanol, pentanol, hexanol, heptanol, octanol, butanediol, 3-hydroxybutyrolactone, xylitol, arabinitol, sorbitol, mannitol, vitamin C, riboflavin, thiamine, tocopherol, cobalamin, pantothenic acid, biotin, pyridoxine, niacin, folic acid, 3-hydroxy propionaldehyde, 3-hydroxybutyrolactone, diamino pentane, diamino hexane and dihydroxyacetone. ### Claim 4 (depends on 3) The method of claim 3, wherein the chemical substance is obtained at a purity of at least Technical Grade. ### Claim 5 (depends on 1) The method of claim 1, wherein the L. diolivorans is derived from the DSM 14421 or LMG 19668 or G77 strain. ### Claim 6 (depends on 1) The method of claim 1, wherein the L. diolivorans is a genetically engineered or recombinant strain of L. diolivorans. ### Claim 7 (depends on 6) The method of claim 6, wherein the strain is subjected to mutagenesis and selected for the improved biotransformation. ### Claim 8 (depends on 1) The method of claim 1, wherein the L. diolivorans is cultivated in a batch mode, fed-batch mode or continuous mode. ### Claim 9 (depends on 1) The method of claim 1, wherein the L. diolivorans is first cultured in a batch culture to accumulate L. diolivorans biomass, followed by biotransforming the carbohydrate into the chemical substance in a fed-batch culture. ### Claim 10 (depends on 1) The method of claim 1, wherein (i) the chemical substance is 3-hydroxy propionic acid, the carbohydrate is glycerol, and the mixture of organic carbon sources is industrial glycerol; (ii) the chemical substance is lactic acid, the carbohydrate is cellulose, and the mixture of organic carbon sources is lignocellulose or lignocellulosic biomass hydrolysate; or (iii) the chemical substance is mannitol, the carbohydrate is saccharose, and the mixture of organic carbon sources is sugar beet extract or hydrolysate. ### Claim 11 (depends on 1) The method of claim 1, wherein the L. diolivorans is a wild-type L. diolivorans. ### Claim 12 (depends on 6) The method of claim 6, wherein the genetically engineered or recombinant strain of L. diolivorans overexpresses homologous sequences and/or is transformed with a vector comprising heterologous sequences, to increase the yield of the chemical substance, wherein the strain of L. diolivorans is not a wild-type organism isolated from natural sources.