# US 10,119,148 B2 · RNAs from pathogens inhibit plant immunity

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- Number: US 10,119,148 B2
- Document ID: US10119148B2
- Status: Granted
- Kind: B2
- Application number: 14/505,378
- Filed: 2014-10-02
- Published: 2015-07-23
- Granted: 2018-11-06
- Earliest claimed: 2013-10-02
- Assignees: The Regents of the University of California
- Applicants: The Regents of the University of California
- Inventors: Hailing Jin
- Web page: https://patentgrep.com/patent/US10119148B2

## CPC

- C12N 15/8282: {for fungal resistance}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to pathogen-resistant plants comprising a heterologous expression cassette, the expression cassette comprising a promoter operably linked to a polynucleotide that is complementary to, or mediates destruction, of a plant immunity suppressing sRNA of a pathogen, wherein the plant is less susceptible to the pathogen compared to a control plant lacking the expression cassette. Methods of making and cultivating pathogen-resistant plants are also provided.

## Claims

### Claim 1

A pathogen-resistant plant comprising a heterologous expression cassette, the expression cassette comprising a promoter operably linked to a polynucleotide that is complementary to a plant immunity suppressing small RNA (sRNA) of a Botrytis pathogen or a polynucleotide that encodes a short tandem target mimic (STTM) of the sRNA, wherein the sRNA comprises the sequence set forth in SEQ ID NO:58 and wherein the plant is less susceptible to the pathogen compared to a control plant lacking the expression cassette.

### Claim 2 (depends on 1)

The pathogen-resistant plant of claim 1, wherein the pathogen is Botrytis cinerea.

### Claim 3 (depends on 1)

The pathogen-resistant plant of claim 1, wherein the polynucleotide encodes a STTM of the sRNA.

### Claim 4 (depends on 1)

The pathogen-resistant plant of claim 1, wherein the polynucleotide encodes an antisense nucleic acid that is complementary to the sRNA.

### Claim 5 (depends on 1)

The pathogen-resistant plant of claim 1, wherein the promoter is an inducible promoter.

### Claim 6 (depends on 5)

The pathogen-resistant plant of claim 5, wherein the promoter is pathogen inducible.

### Claim 7 (depends on 1)

The pathogen-resistant plant of claim 1, wherein the promoter is tissue-specific.

### Claim 8 (depends on 1)

A method of making a pathogen-resistant plant of claim 1, the method comprising introducing a nucleic acid comprising the expression cassette into a plurality of plants; and
selecting a plant comprising the expression cassette.

### Claim 9

An isolated nucleic acid comprising an expression cassette comprising a promoter operably linked to a polynucleotide that is complementary to a plant immunity suppressing small RNA (sRNA) of a Botrytis pathogen or a polynucleotide that encodes a short tandem target mimic (STTM) of the sRNA, wherein the sRNA comprises the sequence set forth in SEQ ID NO:58.

### Claim 10 (depends on 9)

A host cell comprising the nucleic acid of claim 9.

### Claim 11 (depends on 3)

The pathogen-resistant plant of claim 3, wherein the STTM is generated using the primer pair SEQ ID NO:438 and SEQ ID NO:439.

### Claim 12 (depends on 9)

The isolated nucleic acid of claim 9, wherein the polynucleotide encodes a STTM of the sRNA.

### Claim 13 (depends on 12)

The isolated nucleic acid of claim 12, wherein the STTM is generated using the primer pair SEQ ID NO:438 and SEQ ID NO:439.

### Claim 14 (depends on 9)

The isolated nucleic acid of claim 9, wherein the polynucleotide encodes an antisense nucleic acid that is complementary to the sRNA.