# US 10,119,146 B2 · Highly efficient ethanol-fermentative yeast

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,119,146 B2
- Document ID: US10119146B2
- Status: Granted
- Kind: B2
- Application number: 15/532,801
- Filed: 2014-12-05
- Published: 2017-11-30
- Granted: 2018-11-06
- Earliest claimed: 2014-12-05
- Assignees: Honda Motor Co., Ltd.
- Applicants: Honda Motor Co., Ltd.
- Inventors: Yoshiki Tsuchida; Norihiko Tsukagoshi; Ikumi Kurihara; Kosuke Murata
- Web page: https://patentgrep.com/patent/US10119146B2

## CPC

- C12N 15/815: {for yeasts other than Saccharomyces}
- C12N 1/145: Fungi isolates
- C12N 1/165: Yeast isolates
- C12N 1/20: Bacteria; Culture media therefor
- C12P 7/06: Ethanol, i.e. non-beverage
- C12P 7/065: {with microorganisms other than yeasts}
- C12R 2001/645: Fungi {; Processes using fungi}
- C12R 2001/84: Pichia
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

Provided is a highly efficient ethanol-fermentative yeast having high efficiency in ethanol production without introducing a foreign gene. The highly efficient ethanol-fermentative yeast is a fermentative yeast that effectively produces ethanol from pentose and hexose and is deposited to NITE Patent Microorganisms Depositary under the accession number NITE BP-01962.

## Claims

### Claim 1

A highly efficient ethanol-fermentative yeast, the fermentative yeast effectively producing ethanol from pentose and hexose,
wherein the fermentative yeast has xylose utilization and ethanol fermentation performance improved, in comparison with a wild strain of Meyerozyma guilliermondii, by performing habituation of the wild strain of Meyerozyma guilliermondii in culture in a medium in which a mutagen is added to an enzymatically saccharified liquid derived from rice straw treated with ammonia, the mutagen including any one of an ethylating agent, a base analog and a nitroso compound, and selecting yeasts growing in the medium and
wherein the fermentative yeast is deposited to National Institute of Technology and Evaluation (NITE) Patent Microorganisms Depositary under the accession number NITE BP-01962.

### Claim 2

An ethanol-fermentative yeast deposited to National Institute of Technology and Evaluation (NITE) Patent Microorganisms Depositary under accession number NITE ABP-01962.