# US 10,119,130 B2 · Polypeptides having lysozyme activity and compositions comprising it

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- Number: US 10,119,130 B2
- Document ID: US10119130B2
- Status: Granted
- Kind: B2
- Application number: 15/606,031
- Filed: 2017-05-26
- Published: 2017-11-16
- Granted: 2018-11-06
- Earliest claimed: 2011-11-25
- Assignees: Novozymes A/S
- Applicants: Novozymes A/S
- Inventors: Kirk Matthew Schnorr; Jens Erik Nielsen; Mikkel Klausen
- Web page: https://patentgrep.com/patent/US10119130B2

## CPC

- C12N 9/2462: {Lysozyme (3.2.1.17)}
- A23K 20/174: Vitamins
- A23K 20/184: Hormones
- A23K 20/189: Enzymes
- A23K 20/20: Inorganic substances, e.g. oligoelements
- A23K 40/20: by moulding, e.g. making cakes or briquettes
- A23K 40/25: by extrusion
- A23K 50/75: for poultry
- A23K 50/80: for aquatic animals, e.g. fish, crustaceans or molluscs
- A61P 31/04: Antibacterial agents
- C11B 1/102: {in counter-current; utilisation of an equipment wherein the material is conveyed by a screw}
- C11D 3/38636: {containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase}
- C12N 15/1003: {Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor}
- C12Y 302/01017: Lysozyme (3.2.1.17)
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- Y02A 40/818: Alternative feeds for fish, e.g. in aquacultures
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23K: FODDER
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C11B: PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES (drying-oils C09F)
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12Y: ENZYMES
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

## Claims

### Claim 1

A granule comprising a polypeptide having lysozyme activity, wherein the granule comprises a core particle comprising the polypeptide and a coating, and wherein the polypeptide has at least 90% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

### Claim 2 (depends on 1)

The granule of claim 1, wherein the polypeptide has at least 92% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

### Claim 3 (depends on 1)

The granule of claim 1, wherein the polypeptide has at least 95% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

### Claim 4 (depends on 1)

The granule of claim 1, wherein the polypeptide has at least 97% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

### Claim 5 (depends on 1)

The granule of claim 1, wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 3 or SEQ ID NO: 7, or the full-length complement of thereof, and has at least 90% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

### Claim 6 (depends on 1)

The granule of claim 1, wherein the polypeptide is a variant of a polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8, comprising a substitution, deletion, and/or insertion at one or more positions, and has at least 90% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

### Claim 7 (depends on 1)

The granule of claim 1, wherein the polypeptide is a fragment of the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8, which has lysozyme activity and has at least 90% sequence identity to a polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

### Claim 8 (depends on 1)

The granule of claim 1, wherein the polypeptide comprises or consists of amino acids 1 to 208 of SEQ ID NO: 8.

### Claim 9 (depends on 1)

A detergent composition comprising the granule of claim 1 and a surfactant.

### Claim 10 (depends on 9)

The detergent composition of claim 9, which further comprises one or more enzymes selected from the group consisting of amylases, catalases, cellulases, cutinases, haloperoxygenases, lipases, mannanases, pectinases, pectin lyases, peroxidases, proteases, xanthanases, and xyloglucanases, or any mixture thereof.

### Claim 11 (depends on 9)

The detergent composition of claim 9, further comprising one or more components selected from the group consisting of builders, hydrotopes, bleaching systems, polymers, fabric hueing agents, adjunct materials, dispersants, dye transfer inhibiting agents, fluorescent whitening agents, soil release polymers and anti-redeposition agents.

### Claim 12 (depends on 1)

An animal feed composition comprising the granule of claim 1.

### Claim 13 (depends on 12)

The animal feed composition of claim 12, which further comprises one or more enzymes selected from the group consisting of amylases; galactanases; alpha-galactosidases; beta-glucanases, phospholipases, phytases; proteases, and xylanases; or any mixture thereof.

### Claim 14 (depends on 1)

An animal feed additive comprising
at least one granule of claim 1; and
at least one fat-soluble vitamin, and/or
at least one water-soluble vitamin, and/or
at least one trace mineral.

### Claim 15 (depends on 14)

The animal feed additive of claim 14, which further comprises one or more enzymes selected from the group consisting of amylases; galactanases; alpha-galactosidases; beta-glucanases, phospholipases, phytases; proteases, and xylanases; or any mixture thereof.