Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof
AssigneesLos Alamos National Security, LLC, University of Maine System Board of Trustees
InventorsPat J Unkefer, Penelope S Anderson, Thomas J Knight
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Abstract
Glutamine phenylpyruvate transaminase (GPT) proteins, nucleic acid molecules encoding GPT proteins, and uses thereof are disclosed. Provided herein are various GPT proteins and GPT gene coding sequences isolated from a number of plant species. As disclosed herein, GPT proteins share remarkable structural similarity within plant species, and are active in catalyzing the synthesis of 2-hydroxy-5-oxoproline (2-oxoglutaramate), a powerful signal metabolite which regulates the function of a large number of genes involved in the photosynthesis apparatus, carbon fixation and nitrogen metabolism.
Claims28
A host cell comprising:
- (a) a glutamine phenylpyruvate transaminase (GPT) nucleic acid construct comprising a heterologous plant promoter operably linked to an isolated polynucleotide encoding a GPT polypeptide having GPT catalytic activity, or a vector comprising the GPT nucleic acid construct;
- and
- (b) a glutamine synthetase (GS) nucleic acid construct comprising a heterologous plant promoter operably linked to an isolated polynucleotide encoding a GS polypeptide having GS catalytic activity, or a vector comprising the GS nucleic acid construct.
Dependencies are inferred from the claim text.