# US 10,119,119 B2 · Polysiloxane substrates with highly-tunable elastic modulus > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,119,119 B2 - Document ID: US10119119B2 - Status: Granted - Kind: B2 - Application number: 14/375,705 - Filed: 2013-01-31 - Published: 2015-01-08 - Granted: 2018-11-06 - Earliest claimed: 2012-01-31 - Assignees: Carnegie Mellon University and University of Pittsburgh-Of the Commonwealth System of Higher Education - Applicants: Carnegie Mellon University; University of Pittsburgh—Of the Commonwealth System of Higher Education - Inventors: Adam Walter Feinberg; James L. Funderburgh; Rachelle Simko - Web page: https://patentgrep.com/patent/US10119119B2 ## CPC - C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)} - A61L 27/18: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds - A61L 27/22: Polypeptides or derivatives thereof {, e.g. degradation products} - A61L 27/446: {with other specific inorganic fillers other than those covered by A61L27/443 or A61L27/46} - B05D 1/286: {using a temporary backing to which the coating has been applied} - B05D 1/36: Successively applying liquids or other fluent materials, e.g. without intermediate treatment - B05D 7/54: {No clear coat specified} - C08L 83/00: Compositions of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon only; Compositions of derivatives of such polymers - C08L 83/04: Polysiloxanes - C12M 23/20: {Material Coatings (immunocoatings C12M25/00)} - C12M 25/14: {Scaffolds; Matrices (in general C12N5/0068)} - G01N 33/56966: {Animal cells} - A61L 2400/12: Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces - C08G 77/12: containing silicon bound to hydrogen - C08G 77/24: halogen-containing groups - C08G 77/26: nitrogen-containing groups - C08K 2201/011: Nanostructured additives - C12N 2533/30: Synthetic polymers - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - B05D: PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL (conveying articles or workpieces through baths of liquid B65G, e.g. B65G49/02) - C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06) - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C08G: MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS - C08K: Use of inorganic or non-macromolecular organic substances as compounding ingredients (paints, inks, varnishes, dyes, polishes, adhesives C09) ## Abstract A highly tunable bioscaffold is provided, as well as a method of manufacture of the bioscaffold and methods of use of the bioscaffold, for example for drug testing, cell propagation and for optimizing growth of a cell type, for example corneal endothelial cells. ## Claims ### Claim 1 A bioscaffold comprising: a sterile substrate comprising a crosslinked mixture of a first composition comprising a polysiloxane and nanoparticles and a second composition comprising independently a polysiloxane and which does not comprise nanoparticles, and an extracellular matrix (ECM) component deposited on a surface of the crosslinked mixture; and cells grown or cultured on the sterile substrate. ### Claim 2 (depends on 1) The bioscaffold of claim 1 in which the first composition further comprises polydimethyl siloxane, one or more siloxanes other than polydimethyl siloxane, and silica nanoparticles; and the second composition comprises one or both of a polydimethylsiloxane and a dimethyl, methylhydrogen siloxane. ### Claim 3 (depends on 1) The bioscaffold of claim 1, in which the polysiloxane of the first and second compositions independently comprise one or more of a dimethyl siloxane; a diphenylsiloxane; a diethylsiloxane; a trifluoropropyl methyl siloxane; a phenylmethylsiloxane; a copolymer of dimethylsiloxane with one or more of a diphenylsiloxane, a diethylsiloxane, a trifluoropropyl methyl siloxane, and/or a phenylmethylsiloxane; and a aminopropylmethylsiloxane-(dimethylsiloxane). ### Claim 4 (depends on 1) The bioscaffold of claim 1, in which the nanoparticles are silica nanoparticles. ### Claim 5 (depends on 1) The bioscaffold of claim 1, in which the nanoparticles comprise organically-modified silica. ### Claim 6 (depends on 5) The bioscaffold of claim 5, in which the organically-modified silica comprises one or more vinyl and/or alkyl groups. ### Claim 7 (depends on 5) The bioscaffold of claim 5, in which the organically-modified silica comprises one or both of dimethylvinylated silica and trimethylated silica. ### Claim 8 (depends on 1) The bioscaffold of claim 1, in which the polysiloxane of one or both of the first composition and the second composition is dimethylvinyl-terminated. ### Claim 9 (depends on 1) The bioscaffold of claim 1 in which the first composition comprises dimethylvinyl-terminated dimethyl siloxane, dimethylvinylated and trimethylated silica, and tetra(trimethylsiloxy) silane, and the second composition comprises a polydimethylsiloxane and a dimethyl, methylhydrogen siloxane. ### Claim 10 (depends on 1) The bioscaffold of claim 1, having an elastic modulus ranging from greater than 5 kPa to less than 1.72 MPa. ### Claim 11 (depends on 1) The bioscaffold of claim 1, having a mass ratio of the first composition to the second composition of from 50:1 to 1:50. ### Claim 12 (depends on 1) The bioscaffold of claim 1, in which a weight ratio of polysiloxane to nanoparticles is at least 2.5:1. ### Claim 13 (depends on 1) The bioscaffold of claim 1, in which the ECM component is patterned on a surface of the bioscaffold. ### Claim 14 (depends on 1) The bioscaffold of claim 1, in which the ECM component comprises one or more of a glycosaminoglycan; a proteoglycan; a protein; and a glycoprotein. ### Claim 15 (depends on 1) The bioscaffold of claim 1, formed into a three-dimensional structure. ### Claim 16 (depends on 1) The bioscaffold of claim 1, formed as a porous mass, a woven mass of fibers or a non-woven mass of fibers. ### Claim 17 (depends on 1) The bioscaffold of claim 1, in which the cells are selected from the group consisting of a stem cell, a corneal endothelial cell or a progenitor thereof; a muscle cell or a progenitor thereof; a neuronal cell or a progenitor thereof, and a retinal pigment epithelial cell or a progenitor thereof. ### Claim 18 (depends on 10) The bioscaffold of claim 10, having an elastic modulus of between 5 kPa and 50 kPa. ### Claim 19 (depends on 1) The bioscaffold of claim 1, wherein the ECM component comprises one or more of a heparan sulfate, a dermatan sulfate, a chondroitin sulfate, a keratin sulfate, a hyaluronic acid, an aggrecan, a versican, a neurocan, a brevican, a decorin, a perlecan, a collagen, an elastin; a laminin, a fibronectin, a vitronectin, an osteopontin and a fibrinogen. ### Claim 20 (depends on 1) The bioscaffold of claim 1, further comprising a rigid or semi-rigid substrate onto which the sterile substrate is deposited.