# US 10,119,118 B2 · Modified serum-free cell culture medium

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- Number: US 10,119,118 B2
- Document ID: US10119118B2
- Status: Granted
- Kind: B2
- Application number: 15/187,425
- Filed: 2016-06-20
- Published: 2017-05-11
- Granted: 2018-11-06
- Earliest claimed: 2006-09-13
- Assignees: AbbVie Inc.
- Applicants: AbbVie Inc.
- Inventors: Itzcoatl A. Pla; Joseph G. Matuck; John C. Fann; Christof Schulz; Nichole A. Roy; David F. Bruton; James McIntire; Yu-hsiang D. Chang; Thomas Seewoester
- Web page: https://patentgrep.com/patent/US10119118B2

## CPC

- C12N 5/0037: {Serum-free medium, which may still contain naturally-sourced components}
- C07K 16/00: Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K 16/241: {Tumor Necrosis Factors}
- C07K 16/244: {Interleukins [IL]}
- C07K 16/2869: {against hormone receptors (for antibodies against neuromediator receptors C07K16/286)}
- C12N 5/0031: {Serum-free culture media}
- C12N 5/0603: {Embryonic cells (production of embryos, nuclear transfer A01K67/027); Embryoid bodies}
- C07K 2317/14: Specific host cells or culture conditions, e.g. components, pH or temperature
- C07K 2317/21: from primates, e.g. man
- C12N 2500/24: Iron; Fe chelators; Transferrin
- C12N 2500/25: Insulin-transferrin; Insulin-transferrin-selenium
- C12N 2500/30: Organic components
- C12N 2500/32: Amino acids
- C12N 2500/34: Sugars
- C12N 2500/38: Vitamins
- C12N 2500/50: Soluble polymers, e.g. polyethyleneglycol [PEG]
- C12N 2500/60: Buffer, e.g. pH regulation, osmotic pressure
- C12N 2500/74: from fungi, e.g. yeasts
- C12N 2500/76: from plants
- C12N 2500/90: Serum-free medium, which may still contain naturally-sourced components
- C12N 2501/10: Growth factors
- C12N 2501/33: Insulin
- C12N 2510/02: Cells for production
- C12N 2511/00: Cells for large scale production
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.

## Claims

### Claim 1

A serum-free cell culture medium comprising Part A, Part B, and Part C, wherein
a) Part A consists essentially of a modified basal medium, wherein the modified basal medium is JRH 67411;
b) Part B consists essentially of an inorganic iron source; and
c) Part C comprises a recombinant growth factor; a buffer; and osmolarity regulator; an energy source; and at least two different non-animal hydrolysates.