# US 10,119,113 B2 · Systems for isolating and using clinically safe adipose derived regenerative cells > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,119,113 B2 - Document ID: US10119113B2 - Status: Granted - Kind: B2 - Application number: 14/880,891 - Filed: 2015-10-12 - Published: 2016-05-19 - Granted: 2018-11-06 - Earliest claimed: 2001-12-07 - Assignees: Cytori Therapeutics, Inc. - Applicants: Cytori Therapeutics, Inc. - Inventors: Marc H. Hedrick; John K. Fraser; Michael J. Schulzki; Bobby Byrnes; Grace Carlson; Ronda Elizabeth Schreiber; Isabella Wulur - Web page: https://patentgrep.com/patent/US10119113B2 ## CPC - C12M 47/04: {Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04)} - A61B 17/00: Surgical instruments, devices or methods (A61B18/00 takes precedence; dental tools A61C3/00; eye surgery A61F9/007; ear surgery A61F11/20) - A61K 35/28: Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells - C12M 29/04: {Filters; Permeable or porous membranes or plates, e.g. dialysis} - C12M 45/00: {Means for pre-treatment of biological substances} - C12N 5/0667: {Adipose-derived stem cells [ADSC]; Adipose stromal stem cells} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract Systems and methods are described that are used to separate cells from a wide variety of tissues. In particular, automated systems and methods are described that separate regenerative cells, e.g., stem and/or progenitor cells, from adipose tissue. The systems and methods described herein provide rapid and reliable methods of separating and concentrating regenerative cells suitable for re-infusion into a subject. ## Claims ### Claim 1 An automated system for separating and concentrating regenerative cells from tissue comprising: a collection chamber, which is configured to receive: (1) tissue removed from a subject; and (2) disaggregation agents, such that said disaggregation agents may be added to said tissue in an aseptic manner, and configured to separate said regenerative cells from the tissue; a processing chamber connected to the collection chamber to receive disaggregated tissue from the collection chamber, wherein the processing chamber is coupled to the collection chamber via a conduit; a centrifugation device which can connect to the processing chamber, wherein the centrifugation device is configured to concentrate regenerative cells from the disaggregated tissue present in the processing chamber; a programmable processing device capable of communication with and controlling the collection chamber, the processing chamber, the centrifugation device and associated connections; and an optical sensor along the conduit leading out of the collection chamber. ### Claim 2 (depends on 1) The automated system of claim 1, wherein the processing chamber comprises one or more centrifuge chambers. ### Claim 3 (depends on 1) The automated system of claim 1, wherein the processing chamber is constructed of disposable material that meets biocompatibility requirements for intravascular contact as described in an ISO 10993 standard. ### Claim 4 (depends on 1) The automated system of claim 1, wherein the centrifuge device and the programmable processing device are re-usable. ### Claim 5 (depends on 1) The automated system of claim 1, wherein the collection chamber comprises one or more rigid canisters. ### Claim 6 (depends on 1) The automated system of claim 1, wherein the collection chamber comprises one or more flexible bags. ### Claim 7 (depends on 1) The automated system of claim 1, wherein the collection chamber is constructed of disposable material that meets biocompatibility requirements for intravascular contact as defined in an ISO 10993 standard. ### Claim 8 (depends on 1) The automated system of claim 1, comprising automated valves and pumps for collecting said tissue, wherein the processing device is configured to introduce a pre-programmed amount of a washing agent based on the volume of tissue. ### Claim 9 (depends on 1) The automated system of claim 1, further comprising a waste chamber. ### Claim 10 (depends on 1) The automated system of claim 1, wherein the processing device further comprises a user interface for a user to input parameters into the system. ### Claim 11 (depends on 1) The automated system of claim 1, wherein the processing device further comprises a display screen to display instructions that guide a user to input parameters, confirm pre-programmed steps, warn of errors, or combinations thereof. ### Claim 12 (depends on 1) The automated system of claim 1, wherein the collection chamber includes one or more ports for permitting a washing solution to be delivered to the interior of the chamber, and none or more ports for permitting waste and other materials to be directed out from the collection chamber. ### Claim 13 (depends on 1) The automated system of claim 1, wherein the collection chamber is constructed of biocompatible material that can be sterilized. ### Claim 14 (depends on 1) The automated system of claim 1, wherein the processing chamber is constructed of biocompatible material that can be sterilized. ### Claim 15 (depends on 1) The automated system of claim 1, wherein the optical sensor determines that effluent liquid from the tissue collection chamber is clear and devoid of unwanted material. ### Claim 16 (depends on 1) The automated system of claim 1, further comprising a pressure sensor. ### Claim 17 (depends on 1) The automated system of claim 1, further comprising a temperature control device. ### Claim 18 (depends on 1) The automated system of claim 1, further comprising a bar code reading system. ### Claim 19 (depends on 1) A method of separating and concentrating regenerative cells from adipose tissue comprising introducing adipose tissue into the system of claim 1, and processing said adipose tissue so as to obtain a concentrated population of regenerative cells. ### Claim 20 (depends on 17) The method of claim 17, further comprising cryopreserving at least a portion of the separated and concentrated regenerative cells.