# US 10,119,112 B2 · Multi-reactor unit for dynamic cell culture

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- Number: US 10,119,112 B2
- Document ID: US10119112B2
- Status: Granted
- Kind: B2
- Application number: 13/582,068
- Filed: 2011-03-02
- Published: 2013-04-04
- Granted: 2018-11-06
- Earliest claimed: 2010-03-02
- Assignees: Universite Technologie De Compiegne—UTC; Centre National de la Recherche Scientifique (CNRS)
- Applicants: Cécile Legallais; Régis Baudoin; Eric Leclerc; Jean-Matthieu Prot; Patrick Paullier
- Inventors: Cécile Legallais; Régis Baudoin; Eric Leclerc; Jean-Matthieu Prot; Patrick Paullier
- Web page: https://patentgrep.com/patent/US10119112B2

## CPC

- C12M 41/46: {of cellular or enzymatic activity or functionality, e.g. cell viability}
- B01L 3/5025: {for parallel transport of multiple samples}
- C12M 23/12: {Well or multiwell plates (C12M25/04 takes precedence)}
- C12M 23/42: {Integrated assemblies, e.g. cassettes or cartridges}
- C12M 33/06: {for multiple inoculation or multiple collection of samples}
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules}
- B01L 9/527: {for microfluidic devices, e.g. used for lab-on-a-chip}
- B01L 2200/026: Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L 2300/0877: Flow chambers
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

The present invention relates to a unit (1) comprising a microsystem (150) and at least one set of interface connections (16) for the microsystem (150); the microsystem (150) comprising a bottom plate (152) bearing the impression of at least one microstructured dynamic cell culture chamber (15), and a top plate (151), characterized in that the at least one microstructured chamber (15) is fluidically connected at inlet and/or at outlet by a set of connections (16) inserted removably into a hole in the upper plate (151). The present invention also relates to a dynamic cell culture system to this effect.

## Claims

### Claim 1

A box (1) comprising a microsystem (150), a multi-well plate (110), and at least one set of interface connectors (16), the microsystem (150) comprising a lower plate (152) bearing the impression of at least one microstructured chamber of dynamic cellular culture (15), and an upper plate (151),
wherein the at least one set of interface connectors (16) are fixed in holes (11) of the multi-well plate (110) at one end,
wherein the at least one microstructured chamber of dynamic cellular culture (15) is connected fluidically at an inlet and/or at an outlet by the at least one set of interface connectors (16) inserted detachably in holes of the upper plate (151) at the other end, and
wherein the holes of the upper plate (151) and the connectors (16) have matching diameters so as to provide a hermetic junction between the microsystem (150) and the connectors (16).

### Claim 2

The box as claimed in the preceding claim, wherein said box further comprises at least one well (10), the hole (11) is the hole of the at least one well (10), and the microstructured chamber of dynamic cellular culture (15) is connected to said well (10) via one set of the at least one set of interface connectors (16).

### Claim 3

The box as claimed in the preceding claim, wherein the bottom of the well (10) is at an altitude higher than any point of the microstructured chamber of dynamic cellular culture (15).

### Claim 4

The box as claimed in the preceding claim, wherein the one set of interface connectors (16) is fixed to the hole (11) at the bottom of the well (10).

### Claim 5 (depends on 2)

The box as claimed in claim 2 wherein each of said microstructured chamber of dynamic cellular culture (15) is connected to an inlet well (10a) and an outlet well (10b), and wherein said inlet well (10a) is perfused by a set of inlet connectors (16), and wherein said outlet well (10b) is perfused by a set of outlet connectors (16).

### Claim 6

The box as claimed in the preceding claim, wherein said box comprises a lower casing (100) covered by a sealed hood (200) held by gripping means (20).

### Claim 7

The box as claimed in the preceding claim, wherein the lower casing (100) comprises the microsystem (150) and the multi-well plate (110).

### Claim 8

The box as claimed in the preceding claim, wherein the multi-well plate (110) is a disposable plate made of polystyrene.

### Claim 9 (depends on 7)

The box as claimed in claim 7, wherein the multi-well plate (110) is an autoclavable plate made of polycarbonate.

### Claim 10 (depends on 6)

The box as claimed in claim 6 wherein the sealed hood (200) comprises sets of male connectors (204) perfusing the well (10) and sets of female inlet connectors (201).

### Claim 11

The box as claimed in the preceding claim, wherein said box comprises a male connector (204) and a female connector (201a) per inlet well (10a) and a male connector (204) and two female connectors (201b-c) per outlet well (10b).

### Claim 12 (depends on 1)

The box as claimed in claim 1, wherein the microsystem (150) comprises polydimethylsiloxane.

### Claim 13 (depends on 2)

The box as claimed in claim 2 wherein a membrane insert is arranged in at least one of the wells (10).

### Claim 14 (depends on 1)

A dynamic cellular culture system, comprising
a box (1) as claimed in claim 1, and
at least one fluid circuit (300) comprising circulation piping (310) fitted with circulation means (320) and connected to at least one of said microstructured chamber of dynamic cellular culture (15).

### Claim 15

The dynamic cellular culture system as claimed in the preceding claim, wherein the circulation means (320) is a multichannel peristaltic pump.