Fusion partners for peptide production

A recombinant fusion protein comprising:

• an N-terminal fusion partner, wherein the N-terminal fusion partner is selected from:
• P. fluorescens DnaJ-like protein wherein the P. fluorescens DnaJ-like protein has the amino acid sequence set forth as SEQ ID NO:
• 2;
• P. fluorescens FklB protein wherein the P. fluorescens FklB protein has the amino acid sequence set forth as SEQ ID NO:
• 4, 28, 61, or 62;
• P. fluorescens FrnE protein wherein the P. fluorescens FrnE protein has the amino acid sequence set forth as SEQ ID NO:
• 3, 63, or 64;
• P. fluorescens FkbP protein wherein the P. fluorescens FkbP protein has the amino acid sequence set forth as SEQ ID NO:
• 25;
• and P. fluorescens EcpD protein wherein the P. fluorescens EcpD protein has the amino acid sequence set forth as SEQ ID NO:
• 7, 65, 66, or 67;
• a polypeptide of interest selected from:
• hPTH1-34, a proinsulin that is processed to insulin or an insulin analog;
• Glp1;
• Glp2;
• IGF-1;
• Exenatide (SEQ ID NO:
• 37);
• Teduglutide (SEQ ID NO:
• 39);
• Pramlintide (SEQ ID NO:
• 40);
• Ziconotide (SEQ ID NO:
• 41);
• Becaplermin (SEQ ID NO:
• 42);
• Enfuvirtide (SEQ ID NO:
• 43);
• Nesiritide (SEQ ID NO:
• 44);
• a N-met-GCSF;
• GCSF;
• P. falciparum circumsporozoite protein;
• and IFN-β;
• and
• a linker of 10 to 50 amino acids in length, comprising a cleavage site between the N-terminal fusion partner and the polypeptide of interest.